373276

Hh Signaling Antagonist XIII, HPI-3

The Hh Signaling Antagonist XIII, HPI-3, also referenced under CAS 796887-98-4, controls the biological activity of Hh pathway. This small molecule/inhibitor is primarily used for Cell Signaling applications.

• Synonym(s): Hh Signaling Antagonist XIII, HPI-3, 2-((-(4-Phenylpiperazin-4-yl)quinazolin-4-yl)amino)ethanol
• Empirical Formula (Hill Notation): C₂₀H₂₃N₅O
• CAS Number: 796887-98-4
• Molecular Weight: 349.43
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: beige
• solubility: DMSO: 50 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

A cell-permeable piperazinyl-quinazoline compound that acts as a downstream Hh (Hedgehog) pathway blocker, effectively inhibiting Gli-dependent transcription activity in both Sufu^+/+ NIH 3T3 (shh-LIGHT2) and Sufu^-/- fibroblast cultures (IC₅₀ = 3 & 9 µM, respectively) in a manner similar to two other Hh downstream blockers, HPI-1 (Cat. No. 373275) and GANT-61 (Cat. No. 373401). However, unlike HPI-1, HPI-3′s inhibitory activity against Su(fu)^-/- fibroblasts is completely abolished by Gli1 or Gli2 overexpression. While both HPI-1 and HPI-3 are shown to inhibit the ligand-independent Hh pathway activation in SmoM2-expressing NIH 3T3 cultures (IC₅₀ = 2.5 & 1 µM, respectively), only HPI-1, but not HPI-3, is able to effectively suppress the SmoM2-induced Hh pathway activation in primary murine CGNPs (Cerebellar Granule Neuron Precursors) cultures.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Hyman, J.M., et al. 2009. Proc. Natl. Acad. Sci. USA106, 14132.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable