454585

NMDA Receptor NR2B_CT(1292-1304)-TAT

A 13-mer NMDA receptor 2B (NR2B) subunit carboxy terminal (1292-1304) derived peptide that specifically binds DAPK1 in a reversible manner and antagonizes the activated DAPK1-NR2B interaction and the potentiation of NMDAR function

• Synonym(s): NMDA Receptor NR2B_CT(1292-1304)-TAT, H-KKNRNKLRRQHSY-Ahx-YGRKKRRQRRR-NH₂, Tat-NR2B _CT
• Empirical Formula (Hill Notation): C₁₄₃H₂₅₄N₆₄O₃₂
• Molecular Weight: 3381.96
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: white
• solubility: water: 50 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A 13-mer NMDA receptor 2B (NR2B) subunit carboxy terminal (1292-1304) derived peptide that specifically binds DAPK1 (death-associated protein kinase 1) in a reversible manner and antagonizes the activated DAPK1-NR2B interaction and the potentiation of NMDAR function with no effect on either the catalytic activity of DAPK1 or the NMDA receptor physiology. Blocks Ca²⁺ influx through extrasynaptic NMDA receptors (at 50 µM) and the blood-brain-barrier permeable version, namely NR2B_CT(1292-1304) attached to TAT(47-57) via a spacer (6-aminohexanoic acid) protects cortical neurons from NMDA receptor-mediated insults and reduces brain infarction and improve neurological functions in mice (10 mg/kg, i.v.).

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H₂N-Lys-Lys-Asn-Arg-Asn-Lys-Leu-Arg-Arg-Gln-His-Ser-Tyr-Ahx-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-CONH₂

Physical form

Supplied as a trifluoroacetate salt

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Tu, W., et al. Cell140, 222.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable