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5.08741

Millipore

DAPI Stain - CAS 28718-90-3 - Calbiochem

A 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl in H₂O.

Synonym(s):
DAPI Stain - CAS 28718-90-3 - Calbiochem, 4ʹ,6-Diamidino-2-phenylindole, 2HCl, 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine, 2HCl, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide, 2HCl, DAPI, ≥97% by HPLC, in H₂O
Empirical Formula (Hill Notation):
C16H15N5 · 2HCl
CAS Number:
Molecular Weight:
350.25
MDL number:

assay

≥97% (HPLC)

Quality Level

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles
protect from light

fluorescence

λex ~359 nm
λem ~461 nm

storage temp.

−20°C

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

General description

Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
Cells are typically stained at 1 µg/ml.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
DNA
Reversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Physical form

A 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl (Cat. No. 268298) in H₂O.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

Other Notes

Mathieu, C., et al. 1995. Exp. Cell Res.216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C.105, 251.
Hotz, M.A., et al. 1994. Cytometry15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods26, 249.
Miller, T., et al. 1993. Biotechniques15, 1042.

Gray, J.W., et al. 1982. Science238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods38, 315.

Russels, W.C., et al. 1975. Nature253, 461.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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