530007

Prionogenesis inhibitor, DAPH-12

The Prionogenesis inhibitor, DAPH-12, also referenced under CAS 145915-63-5, blocks the de novo fibrillization process and disassembling preformed NM fibers. This small molecule/inhibitor is primarily used for Inflammation/Immunology applications.

• Synonym(s): Prionogenesis inhibitor, DAPH-12, Aβ42 Amyloidogenesis Inhibitor
• Empirical Formula (Hill Notation): C₂₂H₁₉N₃O₄
• CAS Number: 145915-63-5
• Molecular Weight: 389.40
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: orange
• solubility: DMSO: 100 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

A cell-permeable 4,5-dianilinophthalimide that antagonizes Aβ42 amyloidogenesis as well as yeast Sup35 N-terminal domain- (aa 1 -124; NM) mediated prionogenesis by blocking the de novo fibrillization process and disassembling preformed NM fibers. Treatment with DAPH-12 greatly reduces the ratio between prion-harboring ([PSI⁺]) and non-prion-harboring ([psi^-]) yeast populations. DAPH-12 is also shown to effectively convert the [PSI⁺] phenotype into that of [psi^-] in a dose-dependent manner, while exhibiting no activity against [RNQ⁺] prion-containing cells. While DAPH-12 is in general more effective than EGCG (Cat. No. 324880) in antagonizing the various forms of NM prions both in cell-free and cell-based system, these two reagents used in tandem can create a synergistic effect against antiprion activity.

Warning

Toxicity: Standard Handling (A)

Other Notes

Roberts, B.E., et al. 2009. Nat. Chem. Bio.5, 936.
Wang, H., et al. 2008. Proc. Natl. Acad. Sci. USA105, 7159.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable