535142

Protease Inhibitor Cocktail I

Animal-Free, lyophilized, for the inhibition of serine, esterases, cysteine, trypsin-like and metalloproteases. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

• Synonym(s): Animal-Free Protease Inhibitor Mix, Protease inhibitor
• UNSPSC Code: 12352200
• NACRES: NA.77
• Form: lyophilized
• Solubility: water: soluble
• Storage temp.: −20°C

Properties

• Product Name: Protease Inhibitor Cocktail Set I, Animal-Free, The Protease Inhibitor Cocktail Set I, Animal-Free controls the activity of Protease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• solubility: water: soluble
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A cocktail of five protease inhibitors for the inhibition of a broad range of proteases and esterases. Each vial, when reconstituted with 1 ml H₂O, yields a 100X stock solution. When diluted to 1X the cocktail contains 500 µM AEBSF, HCl (Cat. No. 101500), 150 nM Aprotinin (Cat. No. 616371), 1 µM E-64 (Cat. No. 324890), 0.5 mM EDTA, Disodium Salt and 1 µM Leupeptin Hemisulfate (Cat. No. 108975). Available as 1 ml or as a set of 10 x 1 ml.

Application

Protease Inhibitor Cocktail Set I, Animal-Free has been used:

• as a supplement in cell lysis buffer to extract proteins from non-small-cell lung carcinoma (NSCLC) cell lines[1]
• in NP-40 buffer to lyse the cells for sodium dodecyl sulfate-12% polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting[2]
• as a component in lysis buffer to lyse transfected Huh7 cells and to wash the pellets to detect hepatitis B virus (HBV) C protein phosphorylation in the cell[3]

Biochem/physiol Actions

Protease inhibitor cocktails and specific protease inhibitors are valuable resources for scientists involved in proteomic studies, such as protein expression, characterization, biomarker discovery, post-translational modification mapping, protein profiling, and quantitative protein measurement. By adding a protease inhibitor cocktail or specific inhibitor, the integrity of proteins is preserved for subsequent analysis and further sample characterization.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Reconstitute each vial in 1 ml H₂O to yield a 100X stock solution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Pictograms: GHS08,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H314,H332,H373
• Precautionary Statements: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 4 Inhalation - Eye Dam. 1 - Skin Corr. 1A - STOT RE 2 Inhalation
• Target Organs: Respiratory Tract
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3