539569
PP2C, α-Isoform, His•Tag®, Human, Recombinant, E. coli
Synonym(s):
PPM1A, Protein Phosphatase 2Cα
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About This Item
UNSPSC Code:
12352202
Assay
≥95% (SDS-PAGE)
Quality Level
form
liquid
specific activity
≥8,000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
color
clear colorless
shipped in
wet ice
storage temp.
−70°C
General description
Recombinant, human PP2Cα fused to a His•Tag sequence and expressed in E. coli. A Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response, gene expression and replication in eukaryotes. Recently it has been shown that PP2Cα inhibits cell growth and activates the p53 pathway.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of pNPP per min at 37°C, pH 7.4 using 10 mM of substrate.
Physical form
In PBS, pH 7.4.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Ofek, P., et al. 2003. J. Biol. Chem.278, 14299.
Das, A.K., et al. 1996. EMBO J.15, 6798.
Das, A.K., et al. 1996. EMBO J.15, 6798.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Thuy-Ai Nguyen et al.
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George G Schweitzer et al.
Journal of applied physiology (Bethesda, Md. : 1985), 113(12), 1852-1861 (2012-09-01)
Prior exercise by rats can induce a sustained increase in muscle Akt substrate of 160 kDa (AS160) phosphorylation on Thr(642) (pAS160(Thr642)). Because phosphorylation of AS160 on both AS160(Thr642) and AS160(Ser588) is important for insulin-stimulated glucose transport (GT), we determined if
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