The minimum concentration specification for this product is 0.10 mg/mL. This value is reported on the lot specific Certificate of Analysis and does vary from lot to lot. A brief review of recent lots reveals a range of 1.0 - 4.0 mg/mL. The Certificate can be accessed in the DOCUMENTATION section under the 'Certificate of Analysis' tab: https://www.sigmaaldrich.com/product/mm/553506m#product-documentation
553506-M
RAP, Human, Recombinant, E. coli
Synonym(s):
Receptor Associated Protein
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50 μG
$503.00
$503.00
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50 μG
$503.00
About This Item
UNSPSC Code:
12352202
$503.00
Please contact Customer Service for Availability
Recommended Products
Assay
≥90% (SDS-PAGE)
Quality Level
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
storage temp.
−70°C
1 of 4
This Item | 236608-M | 344145 | 616374 |
|---|---|---|---|
| assay ≥90% (SDS-PAGE) | assay - | assay ≥85% (SDS-PAGE) | assay ≥95% (SDS-PAGE) |
| form liquid | form liquid | form liquid | form liquid |
| storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition do not freeze | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles |
| storage temp. −70°C | storage temp. 2-8°C | storage temp. −70°C | storage temp. −70°C |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® |
General description
Recombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low density lipoprotein receptor-related protein (LRP) and very low-density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low density lipoprotein receptor (LDLR) and several of its class 2 mutants. Expressed as a GST-fusion protein that is subsequently cleaved with thrombin. Typical concentrations to block LRP or VLDL receptor are 200-300 nM. For antagonism of LDLR, 1 µM is recommended.
Warning
Toxicity: Standard Handling (A)
Physical form
150 mM NaCl, 100 mM Tris-HCl, pH 7.4.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Li, Y., et al. 2002. Biochemistry41, 4921.
Salicioni, A.M., et al. 2002. J. Biol. Chem.277, 16160.
Anderson, O.M., et al. 2001. Biochemistry40, 15408.
Bu, G. 2001. Int. Rev. Cytol.209, 79.
Williams, S.E., et al. 1992. J. Biol. Chem.267, 9035
Salicioni, A.M., et al. 2002. J. Biol. Chem.277, 16160.
Anderson, O.M., et al. 2001. Biochemistry40, 15408.
Bu, G. 2001. Int. Rev. Cytol.209, 79.
Williams, S.E., et al. 1992. J. Biol. Chem.267, 9035
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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