MilliporeSigma
All Photos(1)

560000

Millipore

Sputolysin® Reagent

Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum.

CAS Number:
MDL number:

Quality Level

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

shipped in

ambient

storage temp.

15-25°C

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1

InChI key

VHJLVAABSRFDPM-IMJSIDKUSA-N

Related Categories

General description

Note: 1 set = 20 x 10 ml.
A concentrate of dithiothreitol (DTT) in phosphate buffer. Useful for the isolation of pathogenic or saprophytic bacteria, fungi, yeast, and epithelial cells from sputum and other mucoid fluids.
Concentrate of Dithiothreitol (DTT; Cat. No. 233155) in phosphate buffer, pH 6.5-7.5. Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum. Each vial is sufficient to make 100 ml of working solution.

Packaging

10 ml in Glass bottle
1 set in Glass bottle

Warning

Toxicity: Harmful (C)

Physical form

20 vials each containing 10 ml of concentrated DTT OR 1 vial containing 10 ml of concentrated DTT.

Preparation Note

Dilute the contents of one vial to 100 ml with sterile distilled water. Swirl gently to dissolve any crystals. If the diluted material appears cloudy, the solution may be filtered to remove any insoluble particles. The diluted solution is ~6.5 mM dithiothreitol in 100 mM phosphate buffer, pH 7.0.

Reconstitution

Once a vial is opened the unused concentrate should be purged with an inert gas (e.g. N₂) and refrigerated (4°C). Diluted solutions, stored aseptically under an inert gas, are stable for up to 48 h at 4°C.

Other Notes

Hirsch, S.R., et al. 1969. J. Lab. Clin. Med.74, 346.
Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis.94, 454.
Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
Cleland, W.W. 1964. Biochemistry3, 480.
May, J.R. 1953 (September). Lancet p. 535.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
SPUTOLYSIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service