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648310-M
Tris Base, Molecular Biology Grade
≥99% (perchloric acid titration), solid, pH 7.0-9.0, solid, Molecular Biology Grade
Synonym(s):
Tris(hydroxymethyl)aminomethane, tris(Hydroxymethyl)aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol
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$256.00
About This Item
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Product Name
Tris Base, Molecular Biology Grade, TRIS base is useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
useful pH range
7-9
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
SMILES string
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
grade
Molecular Biology
description
Merck USA index - 14, 9772
assay
≥99% (perchloric acid titration)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
solubility
water: 500 mg/mL
cation traces
heavy metals: ≤0.0005%
absorption
≤0.02 at 280 nm
≤0.03 at 260 nm
foreign activity
RNases, DNases, proteases, none detected
storage temp.
10-30°C
Quality Level
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Related Categories
1 of 4
This Item | 648311 | 648315 | 1.02408 |
|---|---|---|---|
| assay ≥99% (perchloric acid titration) | assay ≥99% dry basis (titration) | assay - | assay - |
| grade Molecular Biology | grade - | grade Molecular Biology | grade - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 300 |
| form solid | form crystalline solid | form liquid | form solid |
| storage condition OK to freeze | storage condition OK to freeze | storage condition OK to freeze | storage condition - |
| solubility water: 500 mg/mL | solubility water: 1 M | solubility - | solubility 678 g/L |
Application
- A Fast and Automated Melanin-bleaching Method for Histopathologic Evaluation of Pigmented Melanoma Tissues.: This study presents an automated melanin-bleaching technique using a Tris-based buffer to enhance histopathologic evaluation of pigmented melanoma tissues, aiding in more accurate diagnoses (Ugolini et al., 2022).
- Improvement of plant protein solubilization and 2-DE gel resolution through optimization of the concentration of Tris in the solubilization buffer.: Highlights the optimization of Tris concentration in solubilization buffers to enhance protein solubilization and gel electrophoresis resolution, crucial for biochemical studies (Cho et al., 2010).
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General description
Legal Information
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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