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650277-M

Sigma-Aldrich

Trypsin, Bovine, MS Grade

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1 SET
$341.00

$341.00

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1 SET
$341.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.25

$341.00

Please contact Customer Service for Availability

grade

Proteomics Grade

Quality Level

100

form

lyophilized

specific activity

≥7500 BAEE units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

foreign activity

Chymostrypsin <3 BTEE units/mg solid

storage temp.

−20°C

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This Item
650279TRYPSEQM-ROEMS0007
Trypsin, Bovine, MS Grade

650277-M

Trypsin, Bovine, MS Grade

Trypsin, Porcine, MS Grade

650279

Trypsin, Porcine, MS Grade

Trypsin Sequencing Grade, modified from bovine pancreas

TRYPSEQM-RO

Trypsin Sequencing Grade, modified

Recombinant Trypsin Dimethlyated Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris

EMS0007

Recombinant Trypsin Dimethlyated

grade

Proteomics Grade

grade

Proteomics Grade

grade

protein sequencing grade

grade

Proteomics Grade

specific activity

≥7500 BAEE units/mg protein

specific activity

≥10,000 U/mg

specific activity

-

specific activity

≥10,000 units/mg protein

form

lyophilized

form

lyophilized

form

lyophilized (salt-free)

form

lyophilized powder

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

storage condition

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Roche

manufacturer/tradename

-

General description

A chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry. A trypsin to protein ratio of 1:100 to 1:20 is recommended for mass spectrometry protocols. Note: 1 SET = 5 X 20 µg of modified trypsin and 1 ml of resuspension buffer.

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Unit Definition

One BAEE unit is defined as the ammount of enzyme required to produce a ΔA253 of 0.001 per min in a reaction volume of 3.2 ml at pH 7.6 at 25°C.
One BTEE unit is defined as the amount of enzyme required to hydrolyze 1 µmol BTEE per min at 25°C, pH 7.8

Reconstitution

Following reconstitution, aliquot and freeze (-°C). Unused stock solutions are stable for up to 2 weeks at -70°C. Avoid freeze/thaw cycles of solution.

Other Notes

Henzel, W.J., et al. 1993. PNAS90, 5011.
Rice, R.H., et al. 1977. Biochim Biophys Acta492, 316.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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