70573

Clonables 2X Ligation Premix - Novagen

One solution for optimal ligation in 15 minutes

• UNSPSC Code: 41116133

Properties

• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: dry ice; wet ice
• storage temp.: −70°C

Description

General description

The Clonables 2X Ligation Premix is a single solution containing optimized concentrations of the highest quality T4 DNA ligase, buffer, stabilizer, and cofactors needed for efficient ligation of any type of compatible DNA ends. The premix is tested for ligation of compatible 2- to 4-base cohesive DNA ends as well as blunt ends and single-base overhangs found on some PCR products. Under most conditionsexcellent ligation is achieved in only 15 minutes; with T/A or U/A overhangs more colonies can be obtained by incubating up to two hours. Although maximal efficiencies are obtained using NovaBlue Singles Competent Cells, this reagent is compatible with transformation of any type of chemically competent cells provided by the user. The high performance, minimal pipetting requirement, and single-addition format make the premix suitable for high-throughput applications.

Packaging

Plastic ampoule

Warning

Toxicity: Irritant

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids