This product removes soluble fragments to isolate the insoluble fraction. It does not solubilize insoluble proteins and does not contain EDTA.
70584-M
BugBuster® Protein Extraction Reagent
Synonym(s):
Protein Extraction Reagent, Protein Extraction Solution
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| Size/SKU | Availability | Price |
|---|---|---|
100 mL | Please contact Customer Service for Availability | $154.00 |
500 mL | Please contact Customer Service for Availability | $598.00 |
1000 mL | Please contact Customer Service for Availability | $1,010.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
41106516
form
liquid
Quality Segment
manufacturer/tradename
Novagen®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
input
bacteria and yeast sample(s)
storage temp.
10-30°C
General description
BugBuster® Protein Extraction Reagent is formulated for gentle disruption of Escherichia coli cell wall to liberate active proteins. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The proprietary formulation utilizes a tris-buffer-based mixture of non-ionic and zwitterionic detergents that is capable of cell wall perforation without denaturing the protein. BugBuster is also useful for the preparation of high-purity inclusion bodies for subsequent purification in instances where expressed proteins are insoluble.
Application
BugBuster® Protein Extraction Reagent has been used:
- to lyse Escherichia coli BLR (DE3) cells for β-glucosidase (BglB) production and purification[1]
- as a cell lysis buffer to treat the synechocystis PCC6803 transconjugants and measure the yellow fluorescent protein (YFP) fluorescence[2]
- in lysis buffer for purification of glutathione S-transferase- heat shock protein family A (Hsp70) member 8 (GST- HSPA8)[3]
Legal Information
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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This Item | |||
|---|---|---|---|
| description - | description - | description Simple extraction of soluble protein from mammalian and insect cells | description Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis |
| storage temp. 10-30°C | storage temp. 2-8°C | storage temp. 10-30°C | storage temp. - |
| manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® |
| storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze | storage condition OK to freeze |
| form liquid | form liquid | form liquid | form liquid |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level - |
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Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Can this buffer be used to extract insoluble proteins for subsequent Ni-NTA purification? Does it contain EDTA?"
1 answer-
Helpful?
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Can this be used to extract membrane proteins?
1 answer-
BugBuster Protein Extraction Reagent is a mixture of non-ionic and zwitterionic detergents optimized for the extraction of soluble proteins. It does not solubilize insoluble proteins in inclusion bodies. BugBuster does not lyse cells but creates openings in the membrane to release proteins, similar to most detergent-based extractions. Traditionally, detergents are used to release membrane proteins into the soluble fraction, although not all membrane proteins will be released. Some may remain in the pellet and require denaturing conditions for release. Highly hydrophobic or membrane-associating proteins are likely to be found in the cell debris pellet. The extraction of membrane proteins using BugBuster has not been directly evaluated. Non-ionic detergents can solubilize some membrane proteins, but the extent to which BugBuster achieves this has not been analyzed. It is expected that some, but not all, membrane proteins would be solubilized using BugBuster, and therefore, its use for membrane protein extraction cannot be guaranteed.
Helpful?
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can we use this for protein extarction of P aeruginosa bacteria for Carb NP test
1 answer-
BugBuster® Protein Extraction Reagent is formulated for gentle disruption of E. coli cell wall. It is not tested for use with other organisms. Suitability would have to be determined experimentally.
Helpful?
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Can I use bugbuster to lysis Mycobacterium tuberculosis(ATCC25177)?
1 answer-
BugBuster® Protein Extraction Reagent is formulated for gentle disruption of E. coli cell wall. It is not tested for use with other organisms. Suitability would have to be determined experimentally.
Helpful?
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