This product removes soluble fragments to isolate the insoluble fraction. It does not solubilize insoluble proteins and does not contain EDTA.
70584-M
BugBuster® Protein Extraction Reagent
Synonym(s):
Protein Extraction Reagent, Protein Extraction Solution
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100 ML
$154.00
500 ML
$598.00
1000 ML
$1,010.00
About This Item
NACRES:
NA.32
UNSPSC Code:
41106516
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form
liquid
manufacturer/tradename
Novagen®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
storage temp.
10-30°C
Quality Level
Related Categories
1 of 4
This Item | 70922 | 71009-M | 71186 |
|---|---|---|---|
| form liquid | form liquid | form liquid | form liquid |
| manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® |
| storage condition OK to freeze | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze | storage condition OK to freeze |
| storage temp. 10-30°C | storage temp. 2-8°C | storage temp. 10-30°C | storage temp. - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level - |
General description
BugBuster® Protein Extraction Reagent is formulated for gentle disruption of Escherichia coli cell wall to liberate active proteins. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The proprietary formulation utilizes a tris-buffer-based mixture of non-ionic and zwitterionic detergents that is capable of cell wall perforation without denaturing the protein. BugBuster is also useful for the preparation of high-purity inclusion bodies for subsequent purification in instances where expressed proteins are insoluble.
Application
BugBuster® Protein Extraction Reagent has been used:
- to lyse Escherichia coli BLR (DE3) cells for β-glucosidase (BglB) production and purification[1]
- as a cell lysis buffer to treat the synechocystis PCC6803 transconjugants and measure the yellow fluorescent protein (YFP) fluorescence[2]
- in lysis buffer for purification of glutathione S-transferase- heat shock protein family A (Hsp70) member 8 (GST- HSPA8)[3]
Legal Information
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Patricia Armshaw et al.
Biotechnology for biofuels, 8, 201-201 (2015-12-03)
The use of photosynthetic autotrophs and in particular the model organism Synechocystis PCC6803 is receiving much attention for the production of sustainable biofuels and other economically useful products through metabolic engineering. Optimisation of metabolic-engineered organisms for high-level sustained production of
Dylan Alexander Carlin et al.
PloS one, 12(5), e0176255-e0176255 (2017-05-23)
Accurate modeling of enzyme activity and stability is an important goal of the protein engineering community. However, studies seeking to evaluate current progress are limited by small data sets of quantitative kinetic constants and thermal stability measurements. Here, we report
Tiejian Nie et al.
Autophagy, 17(10), 2923-2938 (2021-05-11)
As a highly dynamic organelle, mitochondria undergo constant fission and fusion to change their morphology and function, coping with various stress conditions. Loss of the balance between fission and fusion leads to impaired mitochondria function, which plays a critical role
Articles
This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.
Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
Related Content
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Les méthodes de lyse cellulaire et d'extraction des protéines couvrent diverses techniques, de la solubilisation par détergent à la disruption mécanique, afin de répondre aux besoins de la recherche.
Handout on Protein Extraction
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Can this buffer be used to extract insoluble proteins for subsequent Ni-NTA purification? Does it contain EDTA?"
1 answer-
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Can this be used to extract membrane proteins?
1 answer-
BugBuster Protein Extraction Reagent is a mixture of non-ionic and zwitterionic detergents optimized for the extraction of soluble proteins. It does not solubilize insoluble proteins in inclusion bodies. BugBuster does not lyse cells but creates openings in the membrane to release proteins, similar to most detergent-based extractions. Traditionally, detergents are used to release membrane proteins into the soluble fraction, although not all membrane proteins will be released. Some may remain in the pellet and require denaturing conditions for release. Highly hydrophobic or membrane-associating proteins are likely to be found in the cell debris pellet. The extraction of membrane proteins using BugBuster has not been directly evaluated. Non-ionic detergents can solubilize some membrane proteins, but the extent to which BugBuster achieves this has not been analyzed. It is expected that some, but not all, membrane proteins would be solubilized using BugBuster, and therefore, its use for membrane protein extraction cannot be guaranteed.
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can we use this for protein extarction of P aeruginosa bacteria for Carb NP test
1 answer-
BugBuster® Protein Extraction Reagent is formulated for gentle disruption of E. coli cell wall. It is not tested for use with other organisms. Suitability would have to be determined experimentally.
Helpful?
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Can I use bugbuster to lysis Mycobacterium tuberculosis(ATCC25177)?
1 answer-
BugBuster® Protein Extraction Reagent is formulated for gentle disruption of E. coli cell wall. It is not tested for use with other organisms. Suitability would have to be determined experimentally.
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