70597

Introductory pETBlue-1 AccepTor Vector Kit - Novagen

• UNSPSC Code: 41106610
• NACRES: NA.54

Properties

• manufacturer/tradename: Novagen^®
• Quality Level: 100
• storage condition: OK to freeze
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

The AccepTor Vector Cloning Kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (i.e. KOD XL polymerase and native and recombinant Taq polymerases), which leave single 3′-dA overhangs on the reaction products. The AccepTor Vectors enable direct ligation by virtue of single 3′-dU overhangs that anneal efficiently with 3′-dA extensions on PCR products. The dU residues are converted to dT residues in vivo following transformation. AccepTor Vectors are supplied in a ready-to-ligate format. Simply mix the vector with your PCR product, add Clonables 2X Ligation Premix (Cat No. 70573), and transform into NovaBlue Singles Competent Cells (Cat. No. 70181). The entire procedure, from PCR product to plating transformants, can be performed in as little as 40 minutes with minimal pipetting. AccepTor Vector Kits are available in an introductory 10-reaction size as well as 20- and 40-reaction configurations. The AccepTor Vectors are also available separately without the ligation and transformation components. Also see pSTBlue-1 AccepTor Vector Giga Kit (Cat. No. 71228) for information on using higher-efficiency competent cells.

Features and Benefits

• No restriction enzymes or special primers
• Direct ligation of PCR product with vector
• Compatible with polymerases that leave single 3´-dA overhangs
• Blue/white screening with pSTBlue-1 or pETBlue-1 vectors
• Simple protocol takes as little as 40 minutes from PCR product to plating transformants

Packaging

Plastic ampoule

Components

• 0.5 µg- pETBlue-1 AccepTor Vector
• 10 µl- AccepTor Vector Positive Control Insert
• 55 µl- Clonables 2X Ligation Mix
• 1.5 ml- Nuclease-free Water
• 11 × 50 µl- NovaBlue Singles Competent Cells
• 3 × 2 ml- SOC Medium
• 10 µl- Test Plasmid
• 0.2 ml- Tuner (DE3) pLacI Competent Cells

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

This product is covered by U.S. Patent 5,856,144 owned by EMD Chemicals Inc. or its Affiliates.