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BugBuster^® (primary amine-free) Extraction Reagent

• Synonym(s): Protein Extraction Reagent

Properties

• form: liquid
• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: ambient
• storage temp.: 10-30°C

Description

General description

BugBuster (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilization or cross-linking. The PIPPS buffer used in BugBuster (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Features and Benefits

Primary Amine-Free Formulation: BugBuster^® (primary amine-free) Extraction Reagent is specially designed for applications where primary amines would interfere with protein extract. This means that academic researchers can purify metal-dependent proteins or proteins to be used for immobilization or crosslinking without worrying about the presence of primary amines, which could cause interference.

Enhanced Extraction: The extraction process can be further enhanced by the addition of rLysozyme Solution and Benzonase Nuclease, which helps to efficiently extract and purify proteins from the sample.

Metal Ion Compatibility: The PIPPS buffer used in the primary amine-free formulation of BugBuster^® has a similar buffer capacity and pH range as the original Tris-buffered BugBuster^® but will not complex metal ions. This makes it an ideal choice for extraction of metal-dependent proteins

Warning

Toxicity: Standard Handling (A)

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable