Skip to Content
MilliporeSigma

71110-M

Millipore

rLysozyme Solution

Stabilized recombinant lysozyme

Synonym(s):

recombinant lysozyme

Slide 1 of 1
Sign Into View Organizational & Contract Pricing

Select a Size

300 KU
$72.70
1200 KU
$173.00
6000 KU
$654.00

$72.70


Please contact Customer Service for Availability

Select a Size

Change View
300 KU
$72.70
1200 KU
$173.00
6000 KU
$654.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

$72.70


Please contact Customer Service for Availability

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

recombinant

expressed in E. coli

Quality Level

form

solution

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAE0152L1667L3790
recombinant

expressed in E. coli

recombinant

-

recombinant

expressed in rice

recombinant

-

form

solution

form

powder

form

lyophilized powder

form

liquid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

-

storage condition

-

storage condition

-

Quality Level

400

Quality Level

100

Quality Level

200

Quality Level

200

manufacturer/tradename

Novagen®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

General description

rLysozyme Solution contains a highlypurified and stabilized recombinant lysozymethat can be used for lysis of Gram-negativebacteria, such as E. coli. The enzyme catalyzes the hydrolysis of N-acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme (1700 KU/mg) for E. coli lysis is 250 times greater than that of chicken egg white lysozyme. rLysozyme is optimally active at physiological pH. Very small amounts of rLysozyme (3–5 KU/g cell paste) enhance the efficiency of protein extraction withBugBuster, BugBuster HT, and PopCultureReagents. In the absence of protein extractionreagents, direct lysis of E. coli can be achieved by treatment of 1.0 gram cell paste with 45–60 KU rLysozyme. The product is supplied as a ready-to-use solution at a concentration range of 27–33 KU/µl in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON X-100, pH 7.5. rLysozyme Solution is stable at –20°C.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit of rLysozyme is defined as the amount of enzyme necessary to cause a decrease of 0.025 A₄₅₀ unit per min at 25°C in a 1-ml suspension (1 mg/ml) of Tuner(DE3) cells in 0.5 x BugBuster diluted with 50 mM Tris-HCl, pH 7.5.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service