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Sigma-Aldrich

pRSFDuet-1 DNA - Novagen

Synonym(s):

pRSFDuet-1 Vector

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10 μG
$600.00

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10 μG
$600.00

About This Item

UNSPSC Code:
41106602
NACRES:
NA.51

$600.00


Please contact Customer Service for Availability

form

liquid

Quality Level

manufacturer/tradename

Novagen®

storage condition

OK to freeze

Promoter

Promoter name: T7

selection

kanamycin

shipped in

wet ice

storage temp.

−70°C

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This Item
711297060871147
promoter

Promoter name: T7

promoter

-

promoter

-

promoter

Promoter name: T7

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−70°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze

General description

pRSFDuet-1 is designed for the co-expression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (RBS). The vector also carries the pRSF1030 replicon (also known as NTP1), LacI gene, and kanamycin resistance marker.

Application

pRSFDuet-1 DNA - Novagen has been used as a vector:
  • to clone the DNA fragments of defensin α 5 (DEFA5) for recombinant expression[1]
  • to clone the coding region of Mad1597–719[2]
  • to clone the globular domain of Bombyx mori Spc24 (73-162aa) with an N-terminal 6X His-tag to generate the B. mori 6xHis-Spc24(73-162)-Spc25(70-211) pRSF-DUET1 bacterial expression constructs[3]

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

  1. Hi, does this plasmid come ready for transformation into cells or does it come lyophilized? Kind regards John

    1 answer
    1. This product is in solution and comes ready for transformation.

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