MilliporeSigma
All Photos(4)

Documents

ABE1859

Sigma-Aldrich

Anti-KAP-1/TRIM28 Antibody

serum, from rabbit

Sign Into View Organizational & Contract Pricing

Synonym(s):
Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, KAP-1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, RING finger protein 96, Tripartite motif-containing protein 28, KAP-1/TRI
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

mouse ... Trim28(21849)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
ABE434SAB4503475MABE432
antibody form

serum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

3B5.1, monoclonal

species reactivity

mouse, human

species reactivity

mouse, human

species reactivity

mouse, human

species reactivity

human

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:20000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

immunocytochemistry: suitable, western blot: suitable

NCBI accession no.

NP_005753

NCBI accession no.

NP_056989

NCBI accession no.

O15164

NCBI accession no.

NP_056990

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Transcription intermediary factor 1-beta (UniProt Q13263; also known as E3 SUMO-protein ligase TRIM28, KAP-1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, Protein phosphatase 1 regulatory subunit 157, RING finger protein 96, TIF1-beta, Transcriptional intermediary factor 1-beta, Tripartite motif-containing 28, Tripartite motif-containing protein 28) is encoded by the TRIM28 (also known as KAP1, PPP1R157, RNF96, TF1B, TIF1B) gene (Gene ID 10155) in human.
Kruppel-associated box-/KRAB-associated protein 1 (KAP-1) is a transcriptional co-repressor that selectively targets the KRAB domain of zinc finger protein-based transcription factors with its N-terminal tripartite RBCC (RING, b-box, coiled-coil) domain. KAP-1 uses its C-terminal PxVxL motif and the PHD-Bromo domain to recruit chromatin modifiers, such as heterochromatin protein 1 (HP-1), Histone-lysine N-methyltransferase SETDB1, nucleosome remodeling and deacetylation (NuRD) complex, and histone deacetylases (HDACs), for transcriptional repression. KAP-1 also plays an essential role in the DNA damage response (DDR). Upon DNA damage, KAP-1 is rapidly phosphorylated by Ataxia telangiectasia mutated (ATM) on Ser824, causing chromatin relaxation to facilitate DNA repair. In addition, SIRT1-mediated KAP-1 deacetylation is reported to stabilize KAP1 and 53BP1 interaction, resulting in an enhancement of NHEJ-mediated repair efficiency in a KAP-1 pSer824-independent manner.

Specificity

Expected to react with both spliced isoforms.

Immunogen

Epitope: Bromo domain
GST-tagged recombinant protein corresponding to the Bromo domain of human KAP-1/TRIM28.

Application

Anti-KAP-1/TRIM28 Antibody is an antibody against KAP-1/TRIM28 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Western Blotting Analysis: A representative lot detected the endogenous KAP-1/Trim28 only in the immunoprecipitats of exogenously expressed FLAG-SirT1 constructs that contain the KAP-1/Trim28-binding domain (a.a. 143-213) using various transfected HEK293T cells (Lin, Y.H., et al. (2015). PLoS One. 10(4):e0123935).
Western Blotting Analysis: A representative lot detected similar level of endogenous KAP-1/Trim28 in HEK293T cells regardless of ionizing radiation treatment or shRNA-mediated SirT1 downregulation (Lin, Y.H., et al. (2015). PLoS One. 10(4):e0123935).
Western Blotting Analysis: A representative lot detected KAP-1/Trim28 in whole cell lysates, but not in BAP1, FoxK1, or FoxK2 immunoprecipitate (Okino, Y., et al. (2015). J. Biol. Chem. 16;290(3):1580-1591).
Immunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated KAP-1/Trim28 from HEK293T cell lysates (Courtesy of Dr. Zhenkun Lou, Mayo clinic, Rochester, MN).
Immunocytochemistry Analysis: An 1:1000 dilution from a representative lot detected KAP-1/Trim28 nuclear localization in U2OS cells (Courtesy of Dr. Zhenkun Lou, Mayo clinic, Rochester, MN).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated SirT1 with KAP-1/Trim28 from HEK293T cell lysates (Lin, Y.H., et al. (2015). PLoS One. 10(4):e0123935).
Western Blotting Analysis: An 1:1000 dilution from a representative lot detected KAP-1/Trim28 in mouse embryonic fibroblast (MEF) lysate, as well as downregulated KAP-1/Trim28 in lysates from KAP1 siRNA-treated HEK293T cells (Courtesy of Dr. Zhenkun Lou, Mayo clinic, Rochester, MN).

Quality

Evaluated by Western Blotting in HEK293T cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected KAP-1/TRIM28 in 10 µg of HEK293T cell lysate.

Target description

~110 kDa observed

Physical form

Rabbit polyclonal antibody in serum with 0.05% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Lindsay Kosack et al.
Cancer cell, 35(1), 125-139 (2019-01-16)
The marsupial Tasmanian devil (Sarcophilus harrisii) faces extinction due to transmissible devil facial tumor disease (DFTD). To unveil the molecular underpinnings of this transmissible cancer, we combined pharmacological screens with an integrated systems-biology characterization. Sensitivity to inhibitors of ERBB tyrosine

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service