The production was carried out using a modified method as described in the reference: Timpl, R., et al., J. Biol. Chem. 254:9933-9937 (1979). The nidogen content is not tested, but the product is provided at > 95% purity with primarily 3 chains expressed as two major bands at 200 and 400kD, indicating that the presence of nidogen is likely minuscule, if present at all.
CC095-M
Mouse Laminin
from Engelbreth-Holm-Swarm murine sarcoma basement membrane, liquid, 1 mg/mL, suitable for cell culture
Synonym(s):
Purified Laminin derived for mouse
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$332.00
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
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Product Name
Laminin, mouse purified, Laminins are the major noncollagenous constituent of basement membranes. This purified mouse laminin was isolated from Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a mouse tumor that produces large amounts of basement membranes.
biological source
mouse
Quality Level
form
liquid
manufacturer/tradename
Chemicon®
concentration
1 mg/mL
technique(s)
cell culture | mammalian: suitable
input
sample type: human embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type epithelial cells
sample type: mouse embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
1 of 4
This Item | 11243217001 | CLS354259 | AG56P |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell attachment: suitable, cell differentiation: suitable | technique(s) cell culture | mammalian: suitable |
| biological source mouse | biological source mouse (Engelbreth-Holm-Swarm (EHS) sarcoma) | biological source mouse (High Concentration) | biological source human |
| concentration 1 mg/mL | concentration - | concentration - | concentration 0.25 mg/mL |
| Quality Level 100 | Quality Level 100 | Quality Level - | Quality Level 100 |
| form liquid | form solution | form liquid | form liquid |
| shipped in dry ice | shipped in - | shipped in - | shipped in dry ice |
General description
Laminin protein is a non-collagenous glycoprotein isolated from Engelbreth-Holm-Swarm (EHS) mouse sarcoma. A laminin protein molecule is composed of three disulfide-linked polypeptides that assemble to form an asymmetrical cross-shaped structure.[1] Purified laminin mouse purified promotes the adhesion and growth of human carcinoma and sarcoma cells, human retinoblastoma cells, liver cells, mouse neuroblastoma cells, mouse embryonal carcinoma cells, and seems to be involved in the development of embryonal tissue. The optimal laminin concentration may vary with the cell type and has to be determined experimentally.
Application
The purified mouse laminin has been used as a coating substrate for culturing and maintaining mouse embryonic stem cell-derived neuronal cultures, providing a suitable surface for cell attachment and growth during in vitro experiments.[2]
Biochem/physiol Actions
Laminin proteins are essential components of the basement membrane which are extracellular matrices holding cells and tissues together. They interact with receptors present in the plasma membrane of cells neighboring basement membranes. This interaction allows laminins to effectively regulate various cellular activities and signaling pathways.[1]
Physical form
Purified from EHS sarcoma according to the method of Timpl, et al.
Purified protein. Liquid; in 0.05 M Tris-HCl, pH 7.4, containing 0.15 M NaCl.
No preservatives.
No preservatives.
Preparation Note
Specificity: Molecular weight = 850 kD; 3 chains expressed as two major bands at 200 and 400 kD on SDS-PAGE.
Preparation: Prepared from Engelbreth-Holm-Swarm {EHS} sarcoma according to the method of Templ et al 1979 {http://www.jbc.org/cgi/reprint/254/19/9933.pdf} Briefly, tumor homogenates were subjected to NaCl extractions and centrifugations. Laminin was found in the 0.5M NaCl fraction. After subsequent salt gradients and further centrifugation steps, the laminin was dialyzed against 2M urea and passed over a column of DEAE, followed by molecular sieve chromatography and concentration by ultrafiltration.
Preparation: Prepared from Engelbreth-Holm-Swarm {EHS} sarcoma according to the method of Templ et al 1979 {http://www.jbc.org/cgi/reprint/254/19/9933.pdf} Briefly, tumor homogenates were subjected to NaCl extractions and centrifugations. Laminin was found in the 0.5M NaCl fraction. After subsequent salt gradients and further centrifugation steps, the laminin was dialyzed against 2M urea and passed over a column of DEAE, followed by molecular sieve chromatography and concentration by ultrafiltration.
Storage and Stability
Store at -20°C for up to six months.
Thaw product carefully at 2-8°C to avoid premature gelling, and do not refreeze. Once thawed. material should be stored at 2-8°C for up to 3 months.
Thaw product carefully at 2-8°C to avoid premature gelling, and do not refreeze. Once thawed. material should be stored at 2-8°C for up to 3 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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The CoA for this product indicates that it is "Purified as laminin-nidogen complex from EHS sarcoma according to the method of Timpl, et al. (1,2)." The inquiry is whether this is a laminin product or a laminin-nidogen complex.
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