GF023

Tumor Necrosis Factor-α Protein, Recombinant human

Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells & certain other target cells.

• Synonym(s): TNF-alpha
• eCl@ss: 32160405
• NACRES: NA.75

Properties

• biological source: human
• Quality Level: 100
• Assay: >97% (SDS-PAGE)
• specific activity: ≥2 x 107 U/mg
• manufacturer/tradename: Chemicon^®
• technique(s): cell culture | mammalian: suitable
• input: sample type hematopoietic stem cell(s)
• NCBI accession no.: NM_000594.2
• UniProt accession no.: P01375
• shipped in: dry ice
• Gene Information: human ... TNF(7124)

Description

General description

Product Source: E.coli

Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. The mature form of human TNF-alpha has 157 amino acid residues. GF023 is a recombinant polypeptide containing the mature form of human TNF-a with a polyhistidine tag at its amino terminal.

Application

For most in vitro applications, TNF-alpha exerts its biological activity in the concentration range of 0.05 to 20.0 ng/mL. Responding cells are (partial list): Tumor cells, certain other cells.

Physical form

Lyophilized from 3mM Tris, pH 8.0.

Storage and Stability

The lyophilized powder, though stable at room temperature, is best stored desiccated at -20°C. Reconstitute with sterile distilled water to a concentration of 0.1-1 mg/mL. Note: Allow the reconstituted vial to sit at room temperature for 2 hours before use. This solution can be diluted into other buffered solutions and stored at 4°C for one week or -20°C for future use. Reconstituted TNF-alpha should be stored in undiluted aliquots at -20°C for up to six months.

Analysis Note

Specific Activity: Chemicon′s human TNF-alpha, is fully biologically active when compared to standards.The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin-D is ≤ 0.05 ng/mL

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3