GF325

IL-10,
Human Recombinant Animal Free

• Synonym(s): Interleukin-10, IL10
• eCl@ss: 32160405
• NACRES: NA.75

Properties

• impurities: <0.1 μg/mg protein endotoxin (<1 EU/μg)
• Quality Level: 100
• UniProt accession no.: P15248
• shipped in: dry ice

Description

General description

IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-α. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. Recombinant human IL-10 is an 18.6 kDa protein of 161 amino acid residues.

Product Source: Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.

Application

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

Quality

The ED50 was determined by the dosedependent co-stimulation (with human IL-4) of MC/9 cells. The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 10^5
units/mg.

Physical form

Product is presented in 20 mM Sodium Phosphate, pH 7.2. and is filtered through a 0.2 micron filter before lyophilization.

Storage and Stability

Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1