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HTS094RTA

Sigma-Aldrich

Ready-to-Assay GAL1 Galanin Receptor Frozen Cells

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.76

$1,080.00


Please contact Customer Service for Availability

description

Cell line type: GPCR Cell Lines
GPCR Class A
protein target GAL1

Quality Level

technique(s)

calcium flux assay: suitable

detection method

fluorometric

General description

Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Galanin is a 29-30 amino acid peptide originally purified from intestine but later found to be abundant in the CNS. A family of 3 GPCRs, GAL1, GAL2 and GAL3, bind to galanin and mediate its biological effects. GAL1 couples to Gi/o to decrease intracellular cAMP levels (Wang et al., 1998). Galanin and its receptors have been implicated in pain, cognition, seizure activity, depression and drug addiction (Hökfelt, 2005). In particular, GAL1 mediates the anticonvulsant activity of galanin. Mice lacking GAL1 are affected by spontaneous seizures, and have increased susceptibility to seizures induced by lithium-pilocarpine treatment and electrical perforant path stimulation (McColl et al., 2006; Mazarati et al., 2004). Millipore′s cloned human GAL1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GAL1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at GAL1.

Components

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.

Disclaimer

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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