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IL029

Sigma-Aldrich

Interleukin-12 Protein, Recombinant human

The recombinant human Interleukin-12 protein (or IL-12 protein) is a potent regulator of cell mediated immune responses & it induces IFN-gamma production by NK & T cells.

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Synonym(s):
IL-12
eCl@ss:
32160405

biological source

human

Quality Level

recombinant

expressed in CHO cells

Assay

>98% (SDS-PAGE and HPLC)

specific activity

≥1.0 x 10E6 U/mg

manufacturer/tradename

Chemicon®

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 ng/μg endotoxin (of IL-12; 1EU/μg)

input

sample type hematopoietic stem cell(s)

UniProt accession no.

shipped in

dry ice

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Interleukin-12 human

Interleukin-6 human recombinant, expressed in HEK 293 cells, suitable for cell culture

Sigma-Aldrich

SCU0001

Interleukin-6 human

recombinant

expressed in CHO cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

assay

>98% (SDS-PAGE and HPLC)

assay

≥97% (SDS-PAGE and N-terminal analysis)

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

specific activity

≥1.0 x 10E6 U/mg

specific activity

-

specific activity

-

specific activity

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 ng/μg endotoxin (of IL-12; 1EU/μg)

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

≤0.1 EU/μg protein (LAL test)

General description

Interleukin-12 (IL-12) is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-gamma producing Th1 cells. Human IL-12 is a disulfide linked heterodimeric protein with a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.
Product Source: CHO cells (Chinese Hamster Ovarian cells)

Application

For most in vitro applications, IL-12 exerts its biological activity in the concentration range of 1.0 to 10.0 ng/mL.

Biochem/physiol Actions

The ED50 as determined by the induction of IFN-g production from NK cells co-stimulated with IL-18 was ≤ 1.0 ng/mL

Physical form

The sterile filtered solution was lyophilized from 0.5X PBS pH 7.2

Storage and Stability

The lyophilized IL-12, though stable at room temperature, is best stored desiccated below 0°C. Reconstitute with sterile water to a concentration of 0.2 mg/mL. Reconstituted IL-12 should be stored in undiluted aliquots at -20°C for up to 6 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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Jiyun Kim et al.
Biomicrofluidics, 9(4), 044109-044109 (2015-09-05)
Fabrication methods for the development of multiplexed immunoassay platforms primarily depend on the individual functionalization of reaction chambers to achieve a heterogeneous reacting substrate composition, which increases the overall manufacturing time and cost. Here, we describe a new type of

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