MilliporeSigma
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IPVH00005

Millipore

Immobilon®-P PVDF Membrane

1 roll, 27 cm x 1.875 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

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Synonym(s):
Western blotting membrane, blotting membrane, transfer membrane
eCl@ss:
32031602
NACRES:
NB.23

material

PVDF membrane
plain filter
white filter

Quality Level

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

27 cm × 1.875 m

pore size

0.45 μm pore size

capacity

160 μg/cm2 (insulin)
215 μg/cm2 adsorption capacity (BSA)
294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

detection method

chemiluminescent
colorimetric
radioactive

shipped in

ambient

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This Item
IPVH20200IPFL00005IPVH00010
feature

hydrophobic

feature

hydrophobic

feature

hydrophobic

feature

hydrophobic

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

dot blot: suitable, western blot: suitable

technique(s)

western blot: suitable

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

capacity

160 μg/cm2 (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

capacity

160 μg/cm2 adsorption capacity (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

capacity

155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)

capacity

160 μg/cm2 adsorption capacity (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

Application

1 roll, 27 cm x 1.875 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Linkage

Replaces: INCP00010

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10-13 - German Storage Class 10 to 13


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Articles

Semi-Dry or Tank Electrotransfer Troubleshooting

The possible causes and potential remedies for poor or inconsistent transfer of proteins from a gel to the Western blot membrane. Optimal transfer conditions may vary depending on the molecular weight of the protein of interest.

Protein Visualization

Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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