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IPVH00010

Immobilon® -P PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane
plain filter
white filter
Detection method:
chemiluminescent
colorimetric
fluorometric
radioactive
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

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Product Name

Immobilon®-P PVDF Membrane, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

material

PVDF membrane
plain filter
white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

27 cm × 3.75 m

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin)
 215 μg/cm2 adsorption capacity (BSA)
 294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

detection method

chemiluminescent
colorimetric
fluorometric
radioactive

shipped in

ambient

Quality Level

400

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IPVH20200IPVH08130IPVH10100
Immobilon®-P PVDF Membrane 1 roll, 27 cm x 3.75 m, 0.45 &#181;m pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

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IPVH00010

Immobilon®-P PVDF Membrane

Immobilon®-P PVDF Membrane 10 sheets, 20 cm x 20 cm, 0.45 &#181;m pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

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IPVH20200

Immobilon®-P PVDF Membrane

Immobilon®-P PVDF Membrane 10 sheets, 8.5 cm x 13.5 cm, 0.45 &#181;m pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Millipore

IPVH08130

Immobilon®-P PVDF Membrane

Immobilon®-P PVDF Membrane 10 sheets, 10 cm x 10 cm, 0.45 &#181;m pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Millipore

IPVH10100

Immobilon®-P PVDF Membrane

material

PVDF membrane, white filter, plain filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

compatibility

for use with Amido black, for use with Colloidal gold, for use with India ink, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Transillumination, for use with CPTS, for use with Ponceau-S red, for use with Coomassie brilliant blue, for use with Toluidine blue

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

detection method

chemiluminescent, fluorometric, colorimetric, radioactive

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

Features and Benefits

Immobilon-P PVDF Membrane:
  • Won′t crack, curl or fracture when cut
  • Low background
  • Superior staining capabilities
  • Can be reprobed multiple times

General description

Immobilon-P transfer membrane is recommended for most Western blotting applications, especially proteins greater than 20 kDa. Immobilon-P membrane has excellent protein retention, high physical strength, and broad chemical compatibility, making it ideal for a variety of staining applications and reprobing in immunodetection methods.

Other Notes

Filter Code: IPVH
Primary Binding Mechanisms: Electrostatic, hydrophobic
Replaces: INCP00010

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Isabelle Robert et al.
The Journal of experimental medicine, 206(5), 1047-1056 (2009-04-15)
Immunoglobulin class switch recombination (CSR) is initiated by DNA breaks triggered by activation-induced cytidine deaminase (AID). These breaks activate DNA damage response proteins to promote appropriate repair and long-range recombination. Aberrant processing of these breaks, however, results in decreased CSR
Effects of HIV protease inhibitors on progression of monocrotaline- and hypoxia-induced pulmonary hypertension in rats.
Gary-Bobo, Guillaume, et al.
Circulation, 122, 1937-1947 (2010)
Pulmonary artery smooth muscle cell senescence is a pathogenic mechanism for pulmonary hypertension in chronic lung disease.
Noureddine, Hibo, et al.
Circulation Research, 109, 543-553 (2011)
Remodeling of VE-cadherin junctions by the human herpes virus 8 G-protein coupled receptor.
Dwyer, J, et al.
Oncogene, 30, 190-200 (2011)
Interactions between Brucella suis VirB8 and its homolog TraJ from the plasmid pSB102 underline the dynamic nature of type IV secretion systems.
Bourg, Gisele, et al.
Journal of Bacteriology, 191, 2985-2992 (2009)
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Antibody recovery and reuse in the SNAP i.d.®2.0 immunodetection system

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

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Questions

  1. What is the recommended blocking agent for PVDF membranes in a western blot, particularly when using Casein? Is casein suitable for PVDF membranes considering its suitability as a blocking agent?

    1 answer

    1. The recommended blocking agent for PVDF membranes in a Western blot, including when using Casein, as per ther Immobilon®P Transfer Membrane user guide is using a blocking buffer called Block-CH buffer (Cat. No. WBAVDCH01) or a blocking agent such as bovine serum albumin, casein, or nonfat dry milk in wash buffer at a concentration of 0.5-5% (w/v).

      Regarding the suitability of Casein as a blocking agent for PVDF membranes, in-house testing has been conducted with IPVH00010 PVDF membrane, and Casein has been found to be suitable. However, it is important to note that users often choose the blocking agent that works best for their specific needs. Therefore, if one prefers to use Casein as a blocking agent with PVDF membrane (IPVH00010), it can be employed as long as it does not interfere with the samples and antibodies being used.

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