MilliporeSigma
All Photos(1)

ISEQ00010

Millipore

Immobilon®-PSQ PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
Western blotting membrane, blotting membrane, transfer membrane
eCl@ss:
32031602

material

PVDF membrane
plain filter
white filter

Quality Level

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

filter L × W

27 cm × 3.75 m

pore size

0.2 μm pore size

capacity

262 μg/cm2 adsorption capacity (insulin)
340 μg/cm2 adsorption capacity (BSA)
448 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

detection method

chemiluminescent
colorimetric
radioactive

shipped in

ambient

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
ISEQ07850ISEQ00005ISEQ10100
feature

hydrophobic

feature

hydrophobic

feature

hydrophobic

feature

hydrophobic

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

dot blot: suitable, western blot: suitable

technique(s)

western blot: suitable

pore size

0.2 μm pore size

pore size

0.2 μm pore size

pore size

0.2 μm pore size

pore size

0.2 μm pore size

capacity

262 μg/cm2 adsorption capacity (insulin), 340 μg/cm2 adsorption capacity (BSA), 448 μg/cm2 adsorption capacity (goat IgG)

capacity

262 μg/cm2 adsorption capacity (insulin), 340 μg/cm2 adsorption capacity (BSA), 448 μg/cm2 adsorption capacity (goat IgG)

capacity

262 μg/cm2 (insulin), 340 μg/cm2 adsorption capacity (BSA), 448 μg/cm2 adsorption capacity (goat IgG)

capacity

262 μg/cm2 adsorption capacity (insulin), 340 μg/cm2 adsorption capacity (BSA), 448 μg/cm2 adsorption capacity (goat IgG)

General description

The Immobilon®-PSQ membrane is a 0.2 μm microporous membrane made up of polyvinylidene fluoride (PVDF). It has been developed to maximize protein binding. Immobilon®-PSQ membrane is recommended for direct protein sequencing and immunodetection following electroblotting of protein from electrophoresis gels (or dot binding of purified protein samples), especially for proteins with molecular weights less than 20,000. This membrane is compatible with tank and semi-dry electroblotting systems.

Application

Immobilon®-PSQ PVDF Membrane has been used in western blot.

Features and Benefits

Immobilon®-PSQ Membrane:
  • 0.2 μm pore size and a large internal structure
  • Higher protein adsorption and sequencing yields than other membranes
  • Prevents blow-through of low molecular weight proteins

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Chenggong Sun et al.
Journal of hematology & oncology, 13(1), 9-9 (2020-02-02)
PARP inhibitors have been the most promising target drugs with widely proven benefits among ovarian cancer patients. Although platinum-response, HR-related genes, or HRD genomic scar detection are acceptably used in assessment of Olaparib response, there are still evident limitations in
Bing Yan et al.
Theranostics, 10(15), 6728-6742 (2020-06-20)
Rational: Ischemic injury of the skeletal muscle remains a serious clinical problem and currently there is no effective therapy. The aim of the present study is to determine whether human umbilical cord mesenchymal stem cells- derived exosomes (UMSC-Exo) could repair
Chenshuo Luo et al.
Journal of oncology, 2020, 3676751-3676751 (2020-05-16)
To verify and evaluate the value of CD40 as a noninvasive biomarker of cisplatin-resistant bladder cancer, we studied the expression of CD40 and the correlation between nucleophosmin (NPM1) and CD40 in cisplatin-resistant bladder cancer. Three cisplatin-resistant bladder cancer cell lines
Carey Wilson et al.
Neuropharmacology, 168, 107998-107998 (2020-02-18)
Traditional monoaminergic treatments of depression frequently exhibit suboptimal tolerability and effectiveness. The 'short' (s) allele variant of 5-HTTLPR is known to compromise transcriptional efficacy of the serotonin transporter (5-HTT) and can reduce treatment response to traditional antidepressants (e.g. selective serotonin
Weihong Niu et al.
Journal of experimental & clinical cancer research : CR, 39(1), 30-30 (2020-02-08)
BRD7 is a tumor suppressor known to inhibit cell proliferation and cell cycle progression and initiate apoptosis in breast cancer. However, the function and underlying molecular events of BRD7 in tumor invasion and metastasis in breast cancer are not fully

Protocols

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service