MAB3120

Anti-Cre Recombinase Antibody, clone 2D8

ascites fluid, clone 2D8, Chemicon^®

• Synonym(s): Anti-Cre Antibody
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 2D8, monoclonal
• species reactivity (predicted by homology): all
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• UniProt accession no.: P06956
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: Escherichia coli ... Cre(2777477)

Description

General description

Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family. Anti-Cre Recombinase Antibody can be used to determine the success of gene manipulations in transgenic animals.

Specificity

Cre recombinase. The epitope is located between aa 77 and 343.

Immunogen

Cre recombinase (aa 77 to 343) fusion protein

Application

Anti-Cre Recombinase Antibody, clone 2D8 is a well characterized monoclonal antibody. Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Western Blot (natural and recombinant protein):
A 1:500-1:5,000 dilution was used in a previous lot. Natural Cre recombinase from Bacteriophage P1 migrates at 38.5 kDa by SDS-PAGE.

Immunofluorescence:
A previous lot of this antibody was used on IF.

Immunohistochemistry:
A 1:500-1:5,000 dilution of a previous lot was used in IH.

ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.

Optimal working dilutions must be determined by the end user.

Target description

38 kDa

Physical form

Ascites fluid. Contains no preservative

Unpurified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
HeLa Nuclear Extract

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable