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MAB3501

Sigma-Aldrich

Anti-Caspase 2 Antibody, clone 10C6

clone 10C6, Chemicon®, from rat

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Synonym(s):
ICH-1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

10C6, monoclonal

species reactivity

human, monkey, canine, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_032982.2
NM_032983.2

UniProt accession no.

P42575

shipped in

wet ice

Gene Information

human ... CASP2(835)

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This Item
MAB3507MAB4709AB1871
Anti-Caspase 2 Antibody, clone 10C6 clone 10C6, Chemicon®, from rat

Sigma-Aldrich

MAB3501

Anti-Caspase 2 Antibody, clone 10C6

Sigma-Aldrich

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MAB3507

Anti-Caspase 2 Antibody, clone 11B4

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MAB4709

Anti-Caspase 9 Antibody, clone 2-23

Sigma-Aldrich

Sigma-Aldrich

AB1871

Anti-Caspase 1 Antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

clone

10C6, monoclonal

clone

11B4, monoclonal

clone

2-23, monoclonal

clone

polyclonal

species reactivity

human, monkey, canine, mouse

species reactivity

mouse, monkey, human, canine

species reactivity

human, mouse

species reactivity

human, rat, mouse

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, flow cytometry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ELISA: suitable, flow cytometry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable

Specificity

MAB3501 antibody recognizes a reactive epitope that lies in the P19 subunit of human Caspase-2 (ICH-1, Nedd-2).

Immunogen

Raised against the P19 subunit of recombinant human Caspase-2 protein.

Application

Anti-Caspase 2 Antibody, clone 10C6 detects level of Caspase 2 & has been published & validated for use in ELISA, FC, IP & WB.
Research Category
Apoptosis & Cancer

Metabolism
Research Sub Category
Caspases

Enzymes & Biochemistry
Western blot: Recommended usage at 5 μg/ml, which has been found to detect endogenous Caspase-2 in 5 x 10E5 HeLa and MCF-7 cells.

Immunoprecipitation: Recommended concentration 1-5 μg/ml FACS: Recommended concentration 1 μg/ml. It is necessary to permeabilize the cell membrane prior to analysis.

EIA: Recommended concentration 0.5-1 μg/ml.

Immunohistochemistry: Use at 10-20 μg/ml. It is necessary to fix the cells in 4% Paraformaldehyde/0.18% Triton-X100 for 10 minutes prior to staining.

Optimal working dilutions must be determined by end user.

Linkage

Replaces: 04-571

Physical form

Format: Purified
Purified rat monoclonal antibody in buffer containing PBS, 0.1% Kathon.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315 - H317 - H319 - H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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M Seervi et al.
Cell death & disease, 2, e207-e207 (2011-09-09)
Mitochondrial cytochrome c (cyt. c) release and caspase activation are often impaired in tumors with Bcl-2 overexpression or Bax and Bak-defective status. Direct triggering of cell death downstream of Bax and Bak is an attractive strategy to kill such cancers.
Grace X Y Zheng et al.
PLoS genetics, 7(5), e1002054-e1002054 (2011-05-17)
MicroRNAs (miRNAs) post-transcriptionally regulate the expression of thousands of distinct mRNAs. While some regulatory interactions help to maintain basal cellular functions, others are likely relevant in more specific settings, such as response to stress. Here we describe such a role
BH3-only protein BIM mediates heat shock-induced apoptosis.
Mahajan, IM; Chen, MD; Muro, I; Robertson, JD; Wright, CW; Bratton, SB
Testing null
Girish Neelakanta et al.
PloS one, 7(3), e33447-e33447 (2012-03-20)
Drosophila melanogaster experience cold shock injury and die when exposed to low non-freezing temperatures. In this study, we generated transgenic D. melanogaster that express putative Ixodes scapularis antifreeze glycoprotein (IAFGP) and show that the presence of IAFGP increases the ability
E Hermel et al.
Cell death and differentiation, 11(4), 424-438 (2004-01-10)
Huntington's disease (HD) is an autosomal dominant progressive neurodegenerative disorder resulting in selective neuronal loss and dysfunction in the striatum and cortex. The molecular pathways leading to the selectivity of neuronal cell death in HD are poorly understood. Proteolytic processing

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