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MABF171E

Sigma-Aldrich

Anti-CD4 (human), PE, clone RPA-T4 Antibody

clone RPA-T4, from mouse, PE

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Synonym(s):
T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD antigen CD4, CD4
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

PE

antibody form

purified antibody

antibody product type

primary antibodies

clone

RPA-T4, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... CD4(920)

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conjugate

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violetFluor® 450

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

RPA-T4, monoclonal

clone

RPA-T4, monoclonal

clone

RPA-T4, monoclonal

clone

RPA-T4, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

technique(s)

flow cytometry: suitable

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable

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General description

The RPA-T4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RPA-T4 antibody is widely used as a phenotypic marker for human CD4 expression, and is cross-reactive with Chimpanzee CD4. This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone OKT4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065).

Application

This Anti-CD4 (human), PE, clone RPA-T4 Antibody is validated for use in Flow Cytometry for the detection of CD4.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg from a representative lot detected CD4 in one million human peripheral blood lymphocytes.

Other Notes

Concentration: Please refer to lot specific datasheet.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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