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MABF422

Sigma-Aldrich

Anti-CD4 Antibody (human), APC-Cy7, clone RPA-T4

clone RPA-T4, from mouse

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Synonym(s):
T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD antigen CD4
eCl@ss:
32160702
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

APC-Cy7

antibody form

purified antibody

antibody product type

primary antibodies

clone

RPA-T4, monoclonal

species reactivity

human

packaging

pkg of 5 μL (0.5 μg/test)

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1κ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD4(920)

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conjugate

APC-Cy7

conjugate

APC-Cy7

conjugate

PE-Cy7

conjugate

PE

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

RPA-T4, monoclonal

clone

RPA-T4, monoclonal

clone

RPA-T4, monoclonal

clone

RPA-T4, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

packaging

pkg of 5 μL (0.5 μg/test)

packaging

pkg of 5 μL (0.5 μg/test)

packaging

pkg of 5 μL (0.5 μg/test)

packaging

-

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General description

The RPA-T4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RPA-T4 antibody is widely used as a phenotypic marker for human CD4 expression, and is cross-reactive with Chimpanzee CD4. This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone OKT4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065).

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
This mouse monoclonal antibody Anti-CD4 (human), APC-Cy7, clone RPA-T4 validated for use in IP, IHC & Flow Cytometry for the detection of CD4.

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg of this antibody detected CD4 in one million human peripheral blood lymphocytes.

Physical form

Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography

Storage and Stability

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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