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MABS1289

Sigma-Aldrich

Anti-T4 Lysozyme (T4L) Antibody, clone 6A6

clone 6A6, from mouse

Synonym(s):
Bacteriophage T4 endolysin, Bacteriophage T4 lysis protein, Bacteriophage T4 lysozyme, Bacteriophage T4 muramidase
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6A6, monoclonal

species reactivity

phage T4

species reactivity (predicted by homology)

all

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

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MABF421MABF578SAB1411554
antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

clone

6A6, monoclonal

clone

RPA-T4, monoclonal

clone

RPA-T4, monoclonal

clone

polyclonal

species reactivity

phage T4

species reactivity

human

species reactivity

human

species reactivity

human

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

western blot: 1 μg/mL

isotype

IgG1κ

isotype

IgG1κ

isotype

IgG1κ

isotype

-

General description

Bacteriophage T4 endolysin (EC 3.2.1.17; UniProt P00720; also known as T4 lysis protein, T4 lysozyme, T4 muramidase, T4L) is encoded by the T4 E gene. T4 lysozyme (T4L) is a 164-a.a. monomeric protein that hydrolyzes peptidoglycan.T4L functions outside the cytoplasmic membrane and starts the phage extracellular phase by liberating virions from the metabolically inert remains of the bacterial host. Similar to glutathione S-transferase (GST) and T7 lysozyme, T4L sequence is commonly utilized to generate soluble recombinant fusions of hydrophobic proteins, such as multitransmembrane proteins, that are otherwise not soluble when expressed entirely in their native sequences. Antibodies against T4L are useful in detecting T4L in T4-infected bacteria hosts and in probing recombinant T4L fusion proteins.

Specificity

Clone 6A6 detects T4L and recombinant T4L fusion proteins.

Immunogen

Recombinant T4L fusion protein.

Application

Anti-T4 Lysozyme (T4L) Antibody, clone 6A6, Cat. No. MABS1289, is a highly specific mouse monoclonal antibody that targets bacteriophage T4 lysozyme (T4L), validated for use in Immunoprecipitation and Western Blotting for the detection of T4L and T4L-tagged fusion proteins.
Immunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated exogenously expressed TL4 fusion protein from 1.5 µg of lysate of transfected cells by an independent laboratory.
Research Category
Epitope Tags & General Use

Quality

Western Blotting Analysis: 0.1 µg/mL of this antibody detected exogenously expressed T4L fusion protein in 0.2 µg of lysate from transfected cells.

Target description

18.69 kDa calculated.

Physical form

Format: Purified
Protein G purified.
Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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