The maximum concentration of ethanol compatible with Microcon® Centrifugal Filter Devices (10k and 30k) is 10%. This concentration should not be exceeded. Short-term exposure to higher concentrations, such as 70% for disinfection, may be utilized, but this is not validated for repeated or prolonged use.
MRCPRT010
Microcon® Centrifugal Filters
NMWCO 10 kDa, Ultracel® regenerated cellulose membrane (low binding), sample volume 0.5 mL
Synonym(s):
Centrifuge concentrator
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About This Item
UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade)
Pore size:
10 kDa NMWCO
Working volume:
0.5 mL
Sterility:
non-sterile
Packaging:
pkg of 100 ea
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parameter
0.5 mL sample volume
material
Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade)
sterility
non-sterile
feature
holdup volume> 10 μL
packaging
pkg of 100 ea
manufacturer/tradename
Microcon®
technique(s)
DNA purification: suitable, RNA purification: suitable, protein extraction: suitable, protein purification: suitable
L
4.50 cm (1.8 in.)
diam.
12.3 mm
filtration area
0.32 cm2
Quality Level
pore size
10 kDa NMWCO
working volume
0.5 mL
shipped in
ambient
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Related Categories
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This Item | |||
|---|---|---|---|
| pore size 10 kDa NMWCO | pore size 30 kDa NMWCO | pore size - | pore size - |
| manufacturer/tradename Microcon® | manufacturer/tradename Microcon® | manufacturer/tradename Microcon® | manufacturer/tradename Microcon® |
| material Ultracel® regenerated cellulose membrane (low binding), polycarbonate device top, silicone O-ring (medical-grade), polyacetal base (membrane support), polypropylene tube (for filtrate/concentrate) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade, dual cycle ethylene oxide (EtO) treated) |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume |
| feature holdup volume> 10 μL | feature holdup volume> 10 μL | feature holdup volume> 10 μL | feature holdup volume> 10 μL |
General description
Filter Code: PL, Ultracel® low binding regenerated cellulose
Filter Type: Ultrafiltration
Simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. With the low-binding Ultracel membrane, Microcon filters offer:
•Typical recoveries of greater than 95%, even for dilute solutions
•Reverse spin to maximize recovery, even in the smallest samples
•Convenient storage of filtrate or concentrated sample in standard microfuge tube
•Concentration factors up to 100X
Features:
•High recovery for small volumes with reverse spin (concentration factor less than 20X)
•Low-binding Ultracel membrane
•Fast processing
•Typical recoveries of greater than 95%, even for dilute solutions
•Reverse spin to maximize recovery, even in the smallest samples
•Convenient storage of filtrate or concentrated sample in standard microfuge tube
•Concentration factors up to 100X
Features:
•High recovery for small volumes with reverse spin (concentration factor less than 20X)
•Low-binding Ultracel membrane
•Fast processing
Application
This 0.5 mL centrifugal filter enables the concentration of biomolecules with a membrane NMWL of 10 kDa. Micron centrifugal filters are ideal for DNA & protein concentration & desalting.
Features and Benefits
Minimum Final Concentrate Volume: 50 µL
Physical form
Color Code: Green
Other Notes
Replaces: 42407
Legal Information
MICROCON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
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Related Content
Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes.
Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. The low nonspecific binding characteristics of the Ultracel® membrane and the other device components, coupled with its medical-grade o-ring seal, allows the device to accommodate several wash steps with minimal sample loss.
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What is the maximum ethanol concentration that Microcon® Centrifugal Filter Devices (10k, 30k) are compatible with?"
1 answer-
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What is the difference between these and amicon filters?
1 answer-
Microcon and Amicon Ultra products are very similar. Both concentrate liquids down to a retentate and a filtrate. Substances bigger than the molecular weight cutoff stay on top of the membrane and are reduced from a larger initial volume to a smaller final volume. Two key differences are: the Microcon can be slightly better to use for substances in lower initial concentrations and when substances are sticky. Higher recovery can occur with Microcon due to the product being physically smaller and thus, less product is lost to the hold up volume of the membrane and plastic sides of the tube.
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