OP120

Anti-c-ErbB3 (Ab-5) Mouse mAb (H3.105.5)

liquid, clone H3.105.5, Calbiochem^®

• Synonym(s): Anti-HER3, Anti-ErbB3, Anti-Erythroblastosis Virus
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: H3.105.5, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... ERBB3(2065)

Description

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma NSO cells (see application references). Recognizes the ~185 kDa c-Erb B3/HER-3 protein.

Recognizes the c-ErbB3/HER 3 protein in T47D cells and breast and prostate carcinoma tissue.

This Anti-c-ErbB3 (Ab-5) Mouse mAb (H3.105.5) is validated for use in FC, Frozen Sections, Immunoblotting, IP, Neutralization Studies, Paraffin Sections for the detection of c-ErbB3 (Ab-5).

Immunogen

Human

recombinant, human ErbB3 extracellular domain

Application

Flow Cytometry (see comments)

Frozen Sections (not recommended)

Immunoblotting (not recommended)

Immunofluorescence (see comments)

Immunoprecipitation (10 µl/mg protein lysate)

Neutralization Studies (see application references)

Paraffin Sections (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 10 mM PBS, 0.2% BSA, pH 7.4.

Reconstitution

For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.

Analysis Note

Positive Control
T47D cells or breast or prostate carcinoma

Other Notes

Chen, X., et al. 1996. J. Biol. Chem.271, 7620.
Carraway, K.L., et al. 1994. J. Biol. Chem.269, 14303.
Ciardiello, F., et al. 1991. Proc. Natl. Acad. Sci. USA88, 7792.
Plowman, G.D., et al. 1990. Proc. Natl. Acad. Sci. USA87, 4905.
Kraus, J.H., et al. 1989. Proc. Natl. Acad. Sci. USA86, 9193.
Shoyab, M., et al. 1989. Science243, 1074.
Kawakami, T., et al. 1986. Mol. Cell. Biol.6, 4195.

Does not cross-react with c ErbB1 (EGFR), c-ErbB2/HER-2/neu or c-ErbB4/HER-4. c-ErbB3/HER-3 is overexpressed in a variety of tumors (e.g., breast, stomach, pancreas and colon). This antibody is particularly well suited for immunoprecipitating the unoccupied receptor. Reported to block neuregulin-1 β growth and signalling effects in ovarian cancer cell lines. This antibody has also been reported to work for flow cytometry and immunofluorescence. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable