PK1008

PhosphoDetect Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb

liquid, Calbiochem^®

• Synonym(s): Anti-Platelet Derived Growth Factor Receptor-β, Phospho-Specific (Tyr⁷⁵¹)
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• does not contain: preservative
• species reactivity: rat, human, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pTyr751)
• Gene Information: human ... PDGFRB(5159)

Description

General description

Rabbit polyclonal antibody purified by protein A and immunoaffinity chromatography. Recognizes the ~190 kDa PDGF receptor-β protein phosphorylated at Tyr⁷⁵¹.

Recognizes the ~190 kDa PDGF receptor-β protein phosphorylated at Tyr⁷⁵¹ in PDGF-stimulated L6, HepG2, and NIH3T3 cells.

This PhosphoDetect Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb is validated for use in FC, Immunoblotting, IP, Paraffin Sections for the detection of PDGFRβ(pTyr⁷⁵¹).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁷⁵¹ phosphorylation site of human PDGF receptor-β, coupled to KLH

Application

Flow Cytometry (1:200)
Immunoblotting (1:1000)
Immunoprecipitation (1:50)
Paraffin Sections (1:50, heat pre-treatment required; see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Reconstitution

Do not aliquot.

Analysis Note

Positive Control
Breast carcinoma and PDGF-treated L6, HepG2, and NIH3T3 cells

Other Notes

Betsholtz, C., et al. 2001. Bioessays23, 494.
Ostman, A. and Heldin, C.H. 2001. Adv. Cancer Res.80, 1.
Ramalingam, K., et al. 1995. Bioorg. Med. Chem.3, 1263.
Panyotou, G. et al. 1992. EMBO J.11, 4261.
Coughlin, S.R., et al. 1988. Prog. Clin. Biol. Res.266, 39.
Deuel, T.F., et al. 1988. Biofactors1, 213.

For staining paraffin sections bring slides to a boil in 1 mM EDTA, pH 8.0 and continue to heat at sub-boiling temperature for 15 min; no cooling is necessary. Wash paraffin sections with 1X TBS, 0.1% Tween^®-20 detergent. For immunoblotting membranes should be incubated with antibody diluted in 1X TBS, 5% BSA, 0.1% Tween-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2