Stericups are not approved for use in direct patient care applications or diagnostic procedures, as they are designed for laboratory use only.
About This Item
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material
PVDF membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing
sterility
sterile; irradiated
product line
Stericup®
feature
hydrophilic
packaging
pack of 12 ea
parameter
max. temp. at 45 °C
description
2.4 mL (hold-up volume)
technique(s)
sterile filtration: suitable
H
319 mm
filter diam.
73 mm
filtration area
40 cm2
Quality Level
diam.
13.5 cm
filter capacity
1000 mL
matrix
Durapore®
pore size
0.22 μm pore size
fitting
inlet funnel, outlet flask (Receiver)
shipped in
ambient
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This Item | S2GVU05RE | S2GVU01RE | S2GVU10RE |
|---|---|---|---|
| material PVDF membrane, polystyrene funnel(s), polystyrene bottle, polystyrene housing | material PVDF membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing | material PVDF membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing | material PVDF membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing |
| sterility sterile; irradiated | sterility sterile; irradiated | sterility irradiated | sterility sterile; irradiated |
| pore size 0.22 μm pore size | pore size 0.22 μm pore size | pore size 0.22 μm pore size | pore size 0.22 μm pore size |
| packaging pack of 12 ea | packaging pack of 12 ea | packaging pk of 12 ea | packaging pack of 12 ea |
| feature hydrophilic | feature hydrophilic | feature hydrophilic | feature hydrophilic |
| fitting inlet funnel | fitting inlet funnel, outlet flask (Receiver) | fitting inlet funnel, outlet flask (Receiver) | fitting inlet funnel, outlet flask (Receiver) |
General description
Stericup® and Steritop® vacuum sterile filtration devices use reliable, high-performance membranes for optimum sterile filtration of laboratory reagents, buffers, and media. These devices combine superior flow rates and throughput with low non-specific binding and a stable, no-tip design.
This Stericup® 0.22 μm pore size polyvinylidene fluoride (PVDF) membrane filter unit combines a 1000 mL bottle-top with a 1000 mL receiver flask and cap for processing and storage.
Application
- Tissue culture media +/- additives
- Buffers
- Biological solutions
Features and Benefits
- The Quick Release filter funnel disconnects from the receiver bottle with just a quarter turn—reducing the likelihood of spillage and making it easier to manipulate on the bench or under the hood.
- Bold product identification on the Steritop® filter funnel plainly displays membrane characteristics, including composition and pore size, for rapid, application-appropriate product selection.
- Click seal cap is flanged and modified to enable a firm grip in wet or dry conditions—and a tactile stop confirms secure closure. Worry less about the possibility of spilling or contamination of sterilized contents.
- Frosted, ample writing surface on the receiver bottle and lighter cap color facilitate clear labelling to improve legibility and reduce unreadable smears.
- Click seal cap rests on the side to avoid risk of contamination.
- Receiver bottles are stackable to save storage space.
Legal Information
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Select the right sterile filter by media, volume, and application.
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Is it possible to use Stericup for sterile filtering of eye drops intended for human use?
1 answer-
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What is the chemical compatibility of the various Stericup® and Steritop® filters?
What is the chemical compatibility of the various Stericup® and Steritop® filters?
1 answer-
Please refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf
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Can Stericup® and Steritop® filters be used to prepare solutions that will be administered to patients?
1 answer-
No, all Stericup® and Steritop® devices are Research Use Only.
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Can Stericup® bottles be placed inside freezers for long-term storage of media?
1 answer-
You can successfully freeze and store many aqueous solutions (such as culture media) in Stericup® bottles at temperatures up to -20 °C (-4 °F). It is strongly recommended that you run a sample stability trial under your actual storage conditions prior to using Stericup® bottles for frozen storage.
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What is the maximum processing volume for Stericup® and Steritop® filters? Can I add more volume after initial filtration?
1 answer-
All Stericup® and Steritop® filters were developed for single-use application. The maximum volume is dependent on the receiver bottle capacity. Stericup® filters come with a receiver bottle with a specified maximum volume. For Steritop® filters, the maximum recommended volume is 2 L with an appropriate-sized receiver bottle. Larger processing volumes can result in membrane fouling, increase chance of spillage and contamination, and lead to possible airlock*.
*Note that devices with PES membranes can airlock if filtered to dryness. Some liquid must be left on top of the filter, then the system stopped, receiver changed or emptied, and more sample volume added before restart. This increases the chance of spillage.Helpful?
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What tubing can be used with Stericup® and Steritop® vacuum filters?
What tubing can be used with Stericup® and Steritop® vacuum filters?
1 answer-
Stericup® and Steritop® filters are fitted with a 1/4” tube adapter. We recommend 3/16 in. (4.8 mm) ID silicone tubing to fit this tube adapter: https://www.sigmaaldrich.com/product/mm/xx7100004
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Can Stericup® and Steritop® filters be reused?
1 answer-
All Stericup® and Steritop® filters were developed for single use, disposable processing.
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What leachables are detected during filtration processes using Stericup® filters?
1 answer-
We have not performed extractables/leachables studies for our Stericup® filters.
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Can we filter oil-type liquids with Stericup® filters?
Can we filter oil-type liquids with Stericup® filters?
1 answer-
Likely not, due to pore size/possible fouling/chemical compatibility. Refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf
Helpful?
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The User Guide says “Use only glass or plastic bottles designed for vacuum applications”. Can you specify which bottles are compatible?
1 answer-
Steritop® Quick Release bottle top units require a vacuum-rated receiver bottle with either a GL33 or GL45 mm closure thread as specified. Steritop® E bottle top units require a vacuum-rated receiver bottle with a GL45 mm closure thread. Bottles no larger than 2 L should be used.
Glass bottles, GL33:
• Wheaton media bottles (glass), 500 mL (Product Z364886)
• Wheaton media bottles (glass), 1 L (Product Z364894)
Glass bottles, GL45
• Corning® glass media storage bottle, 125 mL (Product CLS1399125)
• Corning® glass media storage bottle, 250 mL (Product CLS1399250)
• Corning® glass media storage bottle, 500 mL (Product CLS1399500)
• Corning® glass media storage bottle, 1 L (Product CLS13991L)
• Pyrex® round media storage bottle, 500 mL (Product CLS1395500)
• Pyrex® round media storage bottle, 1 L (Product CLS13951L)
• KIMBLE® KIMAX® Media bottle, 250 mL (Product DWK14395250)
• KIMBLE® KIMAX® Media bottle, 500 mL (Product DWK14395500)
• KIMBLE® KIMAX® Media bottle, 1 L (Product DWK143951000)
Plastic (pre-sterile) bottles, GL45:
• Stericup® Quick Release Receiver flask, 250 mL (Product S200B02RE)
• Stericup® Quick Release Receiver flask, 500 mL (Product S200B05RE)
• Stericup® Quick Release Receiver flask, 1000 mL (Product S200B10RE)Helpful?
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