SCT029

BioTracker 529 Green Pluripotent Stem Cell Dye

Live cell imaging dye for pluripotent stem cells that selectively identifies undifferentiated human ES and iPS cells.

• Synonym(s): Live cell imaging probe
• UNSPSC Code: 12352207
• NACRES: NA.47
• eCl@ss: 32161000

Properties

• Product Name: BioTracker 529 Green Pluripotent Stem Cell Dye, Live cell imaging dye for pluripotent stem cells that selectively identifies undifferentiated human ES and iPS cells.
• technique(s): cell based assay: suitable
• detection method: fluorometric
• shipped in: ambient

Description

Application

Live cell fluorescent imaging

Research Category
Cell Imaging

Research Sub Category
Live Cell Dye

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Pluripotent stem cells, including embryonic stem cells (ES cells) and induced pluripotent stem cells (iPSCs), have the capacity to give rise to differentiated progeny representative of all three germ layers (ectoderm, endoderm, and mesoderm). Characterization of human iPS cells includes alkaline phosphatase and antibody staining for Oct-4, Sox-2, Nanog, SSEA and TRA surface markers. These assays require cell fixation and are end-point assays.

The BioTracker 529 Green Pluripotent Stem Cell Dye is a live stem cell probe that selectively identifies undifferentiated human ES and iPS cells. The probe localizes at mitochondria in the human stem cells. The dye’s fluorescence is dependent on the activities of ABCB1 and ABCG2 on the cell membrane. The dye is suitable for both live cell imaging and flow cytometry applications of human pluripotent stem cells.

Spectral Properties
Absorbance: 515nm
Emission: 529nm

Physical form

Lyophilized

Preparation Note

Store BioTracker 529 Green Pluripotent Stem Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable