The Fetal Bovine Serum in this product is not heat-inactivated.
SLM-240
RPMI-1640 complete medium
With ʟ-glutamine and 10% US origin FBS, liquid, sterile-filtered, suitable for cell cutlure
Synonym(s):
Low Bicarb Formulation, Roswell Park Memorial Institute 1640 medium
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500 ML
$283.00
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500 ML
$283.00
About This Item
UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.71
Product Name
RPMI 1640 Complete Medium, with 2mM L-Glut and 10% FBS, supplemented with 2mM L-Glutamine and 10% U.S. origin FBS
Quality Level
form
liquid
technique(s)
cell culture | mammalian: suitable
1 of 4
This Item | R5886 | R6504 | R7388 |
|---|---|---|---|
| form liquid | form liquid | form powder | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 300 | Quality Level 400 | Quality Level 500 | Quality Level 400 |
General description
RPMI 1640 CompleteMedium is supplemented with 2mM L-Glutamine and 10% U.S.-origin fetal bovineserum (FBS). This sterile solution is convenient to culture a wide range ofcells that grow in suspension or as anchorage dependent. It can also be used infusion protocols and for growing hybrid cells. Roswell Park Memorial Institute(RPMI) 1640 medium can be used for culturing human normal and neoplasticleukocytes. It supports the growth of various cultured cells, like fresh humanlymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay if theformulation is properly supplemented.
Application
RPMI 1640 CompleteMedium, with 2mM L-Glut and 10% FBS has been used to culture: Jurkatlymphocytes and human U937 monocyte-like cell line(1), HT-29 and SW480 (humancolon cancer cell line) cells(2), monocytes(3)
Quality
pH= 7.2-7.5
Osmolality= 270-292 mOsm/kg
Endotoxin= ≤2 EU/mL
Mycoplasma= Negative
Sterility Tested= Negative for Growth
Appearance= Orange/Red Clear Liquid
Cell Line Tested= A2780 Human Ovarian Carcinoma
Osmolality= 270-292 mOsm/kg
Endotoxin= ≤2 EU/mL
Mycoplasma= Negative
Sterility Tested= Negative for Growth
Appearance= Orange/Red Clear Liquid
Cell Line Tested= A2780 Human Ovarian Carcinoma
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Is the FBS heat-inactivated in Product No. SLM-240-B, RPMI 1640 Complete Medium, with 2mM L-Glut and 10% FBS?
1 answer-
Helpful?
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What is the concentration of NaHCO3, phenol red, sodium pyruvate and HEPES?
1 answer-
The concentration of NaHCO3 is 2.0 g/L and phenol red is 0.0053 g/L. There is not HEPES or sodium pyruvate.
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