Diluting to 500 ul is optional. You have to consider what level of concentration is expected. Amicons can concentrate down to about 15 ul, so a 50 ul sample can only be concentrated ~3.3 fold.
UFC5010
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 10 kDa
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
1 of 2 reviewers took part in a promotion
Synonym(s):
Centrifugal concentrator, 10 kDa MWCO, 0.5 mL sample volume
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8 PKG
$75.20
24 PKG
$196.00
96 PKG
$633.00
500 PKG
$2,540.00
$75.20
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8 PKG
$75.20
24 PKG
$196.00
96 PKG
$633.00
500 PKG
$2,540.00
About This Item
UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24
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$75.20
Please contact Customer Service for Availability
Need it faster? Consider a different package size.
Request a Bulk Order
material
polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
pkg of 500 pkg
pkg of 8 pkg
pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
0.5 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
4.99 cm (1.96 in.)
diam.
10.8 mm
filtration area
1 cm2
volume
0.5 mL
working volume
≤500 μL
0.5 mL
pore size
10 kDa MWCO
greener alternative category
shipped in
ambient
1 of 4
This Item | UFC9010 | UFC8010 | UFC201024 |
|---|---|---|---|
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| pore size 10 kDa MWCO | pore size 10 kDa MWCO | pore size 10 kDa MWCO | pore size 10 kDa MWCO |
| material polypropylene cap, regenerated cellulose membrane, polypropylene tube, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing | material polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing |
| parameter 0.5 mL sample volume | parameter 15 mL sample volume | parameter 4 mL sample volume | parameter 2 mL sample volume |
| product line Amicon® | product line Amicon® | product line Amicon® | product line Amicon® |
General description
Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Application
- Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
Features and Benefits
- Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 500 μL down to 15 μL
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: 10 to 30 minutes
- Reverse spin capability provides consistent recoveries
Physical form
Color Code: Clear
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
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What is the minimum volume allowed by the device? If we have a volume of 50 microliters, is it advisable to dilute it in PBS to obtain a larger volume, closer to 500 microliters?
1 answer-
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What is the pore diameter of Amicon® Ultra Centrifugal Filter, 10 kDa
1 answer-
The ultrafiltration membrane in this product is characterized by a nominal molecular weight limit based on protein retention. An absolute linear pore diameter is not determined. However, a membrane with a 10 kDa molecular weight cutoff will retain nanoparticles of 3-9 nanometers. Please see the related article on nanoparticle ultrafiltration: https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/filtration/nanoparticle-ultrafiltration
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What is the tolerance for molecular weight with these spin columns? Could you reasonably purify a protein of 15 kDa using these columns, or are the tolerances too high and would result in loss of protein into the filtrate?
1 answer-
It is recommended to choose a molecular weight cut-off that is at least two times smaller than the molecular weight of the target molecules.
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Is this filter suitable for purifying MHC-bound peptides as filtrate/eluate while trapping proteins and antibodies as retentate following Immunoprecipitation?
1 answer-
This product is not suitable for separating protein/peptides of different sizes.
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If a centrifuge is unable to achieve a spinning speed of 14000 X g as recommended in the protocol, is it possible to compensate by spinning the samples for a longer duration?
1 answer-
Yes, it is possible to compensate for a lower centrifuge spinning speed by increasing the duration. This can be achieved by calculating the K factor, which determines the adjustment needed in time.
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se puede utilizar los UFC501024 para concentrar una proteina glicosilada?
1 answer-
Yes, Amicon devices like UFC501024 can be used to concentrate any protein greater than about 20 kDa.
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Dear Sir/Mam, can you let me know how many tubes is 8 pkg? Is it 8 tubes? Best regards, Alejandra
1 answer-
It is 8 tubes per pack.
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What is the maximum RPM that this can be spun to ensure the membrane does not perforate?
1 answer-
The recommended G force for the Amicon® Ultra-0.5 filter is 14,000 g & 1,000 g reverse spin when using a Fixed-Angle (35 º) Rotor.
Please refer to the brochure for more information: details: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/260/181/br5002en-protein-prep-workflow-ms.pdf
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