Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 mg | Check Cart for Availability | $104.00 |
About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
Biological source:
bovine pancreas
$104.00
Check Cart for Availability
biological source
bovine pancreas
form
lyophilized
packaging
pkg of 100 mg
manufacturer/tradename
Roche
technique(s)
DNA extraction: suitable
optimum pH
~7.0
storage temp.
2-8°C
General description
Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[1] DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.[2]
Application
Preparation Note
Activator: Ca2+ (0.12 mM) in combination with Mg2+ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.
Other Notes
For life science research only. Not for use in diagnostic procedures.
One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.
1 of 1
This Item | |||
|---|---|---|---|
| biological source bovine pancreas | biological source bovine pancreas | biological source bovine pancreas | biological source - |
| technique(s) DNA extraction: suitable | technique(s) cell culture | mammalian: suitable | technique(s) DNA extraction: suitable | technique(s) RT-PCR: suitable |
| form lyophilized | form lyophilized | form buffered aqueous glycerol solution | form liquid |
| optimum pH ~7.0 | optimum pH - | optimum pH - | optimum pH - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename - | manufacturer/tradename - |
Still not finding the right product?
Explore all of our products under DNase I
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
Alejandro A Pezzulo et al.
Applied and environmental microbiology, 79(19), 5936-5941 (2013-07-23)
Human lungs are constantly exposed to bacteria in the environment, yet the prevailing dogma is that healthy lungs are sterile. DNA sequencing-based studies of pulmonary bacterial diversity challenge this notion. However, DNA-based microbial analysis currently fails to distinguish between DNA
Mitsuteru Natsuizaka et al.
American journal of cancer research, 4(1), 29-41 (2014-02-01)
Insulin-like growth factor binding protein 3 (IGFBP3), a hypoxia-inducible gene, regulates a variety of cellular processes including cell proliferation, senescence, apoptosis and epithelial-mesenchymal transition (EMT). IGFBP3 has been linked to the pathogenesis of cancers. Most previous studies focus upon proapoptotic
M Salimi et al.
Clinical and experimental dermatology, 41(5), 552-556 (2016-01-26)
Studying skin immune cells under various pathophysiological conditions is vital for understanding the nature of cutaneous inflammatory responses. Available methods of isolating cells from the skin have relatively low yield or require in vitro culture. To increase the effective isolation
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 10104159001 | 04061838250445 |