Crude mixture of RNases.Dry powder (prepared from crystallized ribonuclease).
Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.
Heat inactivation: 1 mM EDTA
RNase has been used in DNA ladder assay.
RNase has been used in analysis of DNA fragmentation using agarose gel electrophoresis. It has been used during flow cytometry analysis.
One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under the assay conditions; A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.
Working solution: It is recommended to dissolve the RNase (1 mg/ml) in water or TE buffer, pH 7.5.
For life science research only. Not for use in diagnostic procedures.