MilliporeSigma
All Photos(1)

10109134001

Roche

RNase

from bovine pancreas

Synonym(s):
Rnase

biological source

bovine pancreas

Quality Level

form

dry powder (prepared from crystallized ribonuclease)

specific activity

~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)

packaging

pkg of 500 mg

manufacturer/tradename

Roche

shipped in

wet ice

storage temp.

2-8°C

General description

Crude mixture of RNases.Dry powder (prepared from crystallized ribonuclease).
Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.

Specificity

Heat inactivation: 1 mM EDTA

Application

RNase has been used in DNA ladder assay.
RNase has been used in analysis of DNA fragmentation using agarose gel electrophoresis. It has been used during flow cytometry analysis.

Unit Definition

One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under the assay conditions; A0 to A1 corresponds to the total conversion, A1 being the final absorbance.

Preparation Note

Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.
Working solution: It is recommended to dissolve the RNase (1 mg/ml) in water or TE buffer, pH 7.5.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service