10109886001

Trypsin Inhibitor

from soybean

• Synonym(s): trypsin
• UNSPSC Code: 12352207

Properties

• biological source: soybean
• Quality Level: 100
• form: lyophilized
• mol wt: M_r 20,100
• packaging: pkg of 50 mg
• manufacturer/tradename: Roche
• concentration: 1 mg/mL
• technique(s): cell culture | mammalian: suitable
• pH: 3.8-4.5
• solubility: water: soluble
• storage temp.: 2-8°C

Description

General description

Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges.[1] It belongs to serpins − serine protease inhibitors family.[2]

Specificity

Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases).

Application

Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures.[3]

Trypsin Inhibitor has been used:

• in the buffer for hepatocyte isolation[4]
• to inhibit trypsin in Corneal epithelial cells[5]
• to inhibit papain activity while treating hippocampal neural stem cells (NSCs)[6]

Biochem/physiol Actions

Soybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic.[1] It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin.[2]

Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. The product is sensitive to heat, alkaline pH, and protein-precipitating compounds.

Specifications

Inhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate)

Isoelectric point: 4.5

Unit Definition

1 U = 270 S & T-units or 90 USP-units

Preparation Note

Working concentration: 10 to 100 μg/ml
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.

Reconstitution

Sensitive to heat, alkaline pH, and protein-precipitating compounds.

Storage and Stability

Store at 2 to 8 °C. (Store dry!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable