11271156001

Tumor Necrosis Factor-α, mouse (mTNF-α)

recombinant (E. coli)

• Synonym(s): mTNF, mouse tumor necrosis factor

Properties

• biological source: mouse
• Quality Level: 100
• recombinant: expressed in E. coli
• Assay: >95% (SDS-PAGE)
• form: solution
• potency: <0.0025 ng/mL EC₅₀
• mol wt: 17,200 Da
• packaging: pkg of 2,000,000 U (5 μg, 1ml)
• manufacturer/tradename: Roche
• storage condition: avoid repeated freeze/thaw cycles
• impurities: <1 EU/mgtested (LAL test)
• UniProt accession no.: Q6TDG3
• storage temp.: −20°C
• Gene Information: mouse ... Tnf(21926)

Description

General description

Recombinant murine TNF-α is produced in E. coli and purified by standard chromatographic techniques.
Contents:
5 μg/ml solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane

Specificity

Mouse mTNF-α is effective on mouse, rat, rabbit, and human cells.
Specific activity/EC₅₀: >4 x 108 U/mg, <0.0025 ng/ml (mTNF-a, NIBSC, interim standard, 88/532), at least the same specific activity (EC₅₀) compared to the indicated standard is guaranteed.

Application

TNF-α causes selective necrosis of murine tumors when injected into tumor-bearing mice. In vitro, human TNF-α has direct cytolytic or cytostatic activity on certain transformed cells. In this context, it acts synergistically with interferon-γ. It has also been used for cell stimulation studies.

Quality

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
Note: 1 EU corresponds to 0.1 ng

Specifications

Primary structure: One polypeptide chain (156 amino acids) identical to natural mTNF-a, but not glycosylated. Glycosylation is not essential for biological activity.

Sequence

Chain Length 156 AA

One polypeptide chain (156 amino acids) identical to natural mTNF-α, but not glycosylated. Glycosylation is not essential for biological activity.

Unit Definition

EC₅₀ definition: The amount of mTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.0025 ng/ml).

Preparation Note

Storage conditions (working solution): -15 to -25 °C
The solution can be stored in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): No data available
• Flash Point(C): No data available