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11363743001

Roche

Nutridoma-CS

suitable for hybridoma, solution, pkg of 10 mL (50x)

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Synonym(s):
serum replacements

sterility

sterile; 0.2 μm filtered

Quality Level

form

solution

packaging

pkg of 10 mL (50x)

manufacturer/tradename

Roche

concentration

50 ×

technique(s)

cell culture | hybridoma: suitable

impurities

mycoplasma, tested
100 EU/mL endotoxin (LAL test)

solubility

water: miscible

storage temp.

−20°C

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This Item
F8192F4135H1270
Nutridoma™-CS suitable for hybridoma, solution, pkg of 10 mL (50x)

Roche

11363743001

Nutridoma-CS

Fetal Bovine Serum Australia origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for hybridoma

Sigma-Aldrich

F8192

Fetal Bovine Serum

Fetal Bovine Serum USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

Sigma-Aldrich

F4135

Fetal Bovine Serum

Sigma-Aldrich

Sigma-Aldrich

H1270

Horse Serum

form

solution

form

-

form

-

form

liquid

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

mycoplasma, tested, 100 EU/mL endotoxin (LAL test)

impurities

≤10 EU/mL endotoxin

impurities

≤10 EU/mL endotoxin

impurities

≤10 EU/mL Endotoxin

solubility

water: miscible

solubility

-

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Nutridoma-CS is a serum replacement solution which helps in cell proliferation when added to a suspension. Its major component is β-D xylopyranose which has ring shaped sugar allowing the cells to grow in a suspension even without serum.

Application

Contents
Solution, 50× concentrated, Biochemically defined, filtered through 0.2μm pore size membrane, serum-free medium supplement composed of albumin, insulin, transferrin, several cytokines, a cholesterol source, and other defined organic and inorganic compounds. Nutridoma-CS contains human proteins.
Biochemically defined supplement for the replacement of fetal bovine serum (FBS) in cultures of hybridoma cells. Nutridoma-CS supports the growth of SP2/0-, P3x63Ag8.653-, and NS-1-derived hybridomas. It is specifically formulated to optimize the growth of freshly fused hybridomas during selection and cloning procedures in serum-free cell culture without feeder cells.
Nutridoma-CS has been used as a supplement in neurobasal medium for culturing human embryonic stem cell (hESC) lines.

Unit Definition

Endotoxin (LAL): 100 EU/ml, mycoplasma tested

Physical form

Solution (50x concentrated, pH 7.4); sterile. The protein concentration is less than 1 mg/ml for 2% (1x) working concentration.

Preparation Note

Working concentration: Nutridoma-CS (50x) is diluted 1:50 (v/v) in basal medium. It is strongly suggested to use RPMI 1640. The final protein concentration is less than 1 mg/ml. The final medium should also contain L-glutamine and β-mercaptoethanol.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Nutridoma is a trademark of Roche

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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