MilliporeSigma
All Photos(1)

Documents

11388908910

Roche

Biotin-16-UTP

pkg of 25 μL (250 nmol; 10mM)

All Photos(1)
Synonym(s):
Biotin-16-UTP, biotin

mol wt

Mr 987.5 (biotin-16-UTP-Li4)

Quality Level

100

packaging

pkg of 25 μL (250 nmol; 10mM)

manufacturer/tradename

Roche

storage temp.

−20°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
809608L0289DIGUTP-RO
Biotin-16-UTP pkg of 25 μL (250 nmol; 10mM)

Roche

11388908910

Biotin-16-UTP

Biotin-2′,2′,3′,3′-d4 ≥98 atom % D, ≥95% (CP)

Sigma-Aldrich

809608

Biotin-2′,2′,3′,3′-d4

Lysozyme Biotin-Caproyl lyophilized powder, >20,000 U/mg (Activity of lysozyme used for congugation.)

Sigma-Aldrich

L0289

Lysozyme Biotin-Caproyl

Digoxigenin-11-UTP

Roche

DIGUTP-RO

Digoxigenin-11-UTP

packaging

pkg of 25 μL (250 nmol; 10mM)

packaging

-

packaging

-

packaging

pkg of 25 μL (11209256910 [10 mM]), pkg of 57 μL (03359247910 [3.5 mM])

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Roche

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

General description

For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.

Application

Biotin-labeled RNA can be used as a hybridization probe for:
  • Northern blots
  • Southern blots
  • Plaque or colony lifts
  • RNase protection experiments
  • In situ hybridization
  • Microarray hybridization
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.

Quality

Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).

Physical form

Biotin-16-UTP, tetralithium salt, 10mM solution

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 6

1 of 6

Digoxigenin-11-dUTP, alkali-labile =85% (HPLC), solution, pkg of 25 μL (11573152910 [1 mM]), pkg of 125 μL (11573179910 [1 mM]]])

Roche

DIUTP-RO

Digoxigenin-11-dUTP, alkali-labile

Digoxigenin-11-dUTP, alkali-stable

Roche

DIUTPS-RO

Digoxigenin-11-dUTP, alkali-stable

SP6/T7 Transcription Kit sufficient for 2 x 20 assays (stadard transcription), kit of 1 (12 components), suitable for DNA sequencing, suitable for hybridization

Roche

10999644001

SP6/T7 Transcription Kit

Uridine 5′-triphosphate tris salt Type VI, ≥90%

Sigma-Aldrich

U6875

Uridine 5′-triphosphate tris salt

Aminoallyl-UTP sodium salt ≥75%, lyophilized powder

Sigma-Aldrich

A5660

Aminoallyl-UTP sodium salt

Uridine 5′-triphosphate trisodium salt solution HPLC purified, aqueous solution for RNA polymerase transcription

Sigma-Aldrich

U1006

Uridine 5′-triphosphate trisodium salt solution

Chenying Li et al.
Haematologica, 105(1), 148-160 (2019-04-13)
Homoharringtonine, a plant alkaloid, has been reported to suppress protein synthesis and has been approved by the US Food and Drug Administration for the treatment of chronic myeloid leukemia. Here we show that in acute myeloid leukemia (AML), homoharringtonine potently
Damian LaGamba et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 234(1), 132-142 (2005-07-13)
One of the most fundamental biological processes in development, as well as a primary mechanism for tumor metastasis, is epithelial-mesenchymal transformation (EMT). To gain a greater understanding of this transition, we have obtained a genomic profile of the critical stages
Subramaniam Jayanthi et al.
PloS one, 4(6), e6092-e6092 (2009-07-01)
Methamphetamine (METH) is an illicit toxic psychostimulant which is widely abused. Its toxic effects depend on the release of excessive levels of dopamine (DA) that activates striatal DA receptors. Inhibition of DA-mediated neurotransmission by the DA D1 receptor antagonist, SCH23390
Aikaterini Tsaousi et al.
Circulation research, 108(4), 427-436 (2011-01-05)
Vascular smooth muscle cell (VSMC) proliferation causes intimal thickening in atherosclerosis and restenosis. Previously, we demonstrated that Wnt/β-catenin signaling upregulates VSMC proliferation in vitro. We examined this pathway in vivo and investigated the involvement of specific Wnt proteins in VSMC
Mikail E Abbasov et al.
Nature chemistry, 13(11), 1081-1092 (2021-09-11)
Recent advances in chemical proteomics have begun to characterize the reactivity and ligandability of lysines on a global scale. Yet, only a limited diversity of aminophilic electrophiles have been evaluated for interactions with the lysine proteome. Here, we report an
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service