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PROTAA-RO

Roche

Protein A Agarose

>98% (HPLC and SDS-PAGE), suspension

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Synonym(s):
agarose, protein a

Assay

>98% (HPLC and SDS-PAGE)

form

suspension

packaging

pkg of 15 mL (05015979001 [settled resin volume])
pkg of 2 mL (11719408001 [settled resin volume])
pkg of 5 mL (11134515001 [settled resin volume])

manufacturer/tradename

Roche

capacity

>18 mg/mL binding capacity (human IgG (polyclonal))(the IgG is loaded at pH 7.4 and eluted with 200 mM glycine, pH 2.0)

storage temp.

2-8°C

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This Item
16-125PROTGA-ROP2545
vibrant-m

PROTAA-RO

Protein A Agarose

vibrant-m

16-125

Protein A Agarose

vibrant-m

PROTGA-RO

Protein G Agarose

vibrant-m

P2545

Protein A–Agarose

packaging

pkg of 15 mL (05015979001 [settled resin volume]), pkg of 5 mL (11134515001 [settled resin volume]), pkg of 2 mL (11719408001 [settled resin volume])

packaging

-

packaging

pkg of 15 mL (05015952001 [settled resin volume]), pkg of 2 mL (11719416001 [settled resin volume]), pkg of 5 mL (11243233001 [settled resin volume])

packaging

-

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

form

suspension

form

liquid

form

suspension

form

saline suspension

manufacturer/tradename

Roche

manufacturer/tradename

Upstate®

manufacturer/tradename

Roche

manufacturer/tradename

-

capacity

>18 mg/mL binding capacity (human IgG (polyclonal))(the IgG is loaded at pH 7.4 and eluted with 200 mM glycine, pH 2.0)

capacity

-

capacity

-

capacity

≥15 mg/mL binding capacity (human IgG)

General description

The use of recombinant Protein A in the production of Protein A agarose has the advantage that it is entirely free from Staphylococcal enterotoxins that are known to be mitogenic, and are contaminants in Protein A isolated from S. aureus. The immobilization chemistry provides high IgG-binding capacity and leak-proof stability under a wide range of pH and buffer conditions. The gel can be used over 30 times, depending on the elution condition.

Protein A leakage: <18 ng Protein A/ml (ELISA)
Regeneration: the gel can be used approximately 30 times
Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98% pure, HPLC, SDS-Page)/1 ml gel
Protein A, immobilized.

Specificity

Protein A is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein A binds to varying degrees IgM, IgA, IgD, and most subclasses of IgG.

Application

Purification of mouse monoclonal antibodies from ascites fluid or cell culture supernatants. Protein A or Protein G Agarose affinity matrix can be used to purify antibodies from crude cell extracts and for the immunoprecipitation or co-immunoprecipitation of proteins. It has also been used for photoactivatable-ribonucleoside-enhanced cross-linking immunoprecipitation (PAR-CLIP) analysis.

Components

Preswollen gel, ready-to-use, 2 ml or 5 ml gel (settled gel volume) in buffer that contains 20% ethanol as a preservative, nonsterile.
Protein A content: 3 mg/ml pre-swollen gel

Quality

Purity: >98% (HPLC, SDS-PAGE); entirely free from staphylococcal enterotoxins.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

FlameExclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

102.2 °F

Flash Point(C)

39 °C


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