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X-tremeGENE 360 Transfection Reagent

Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines

transfection reagent


for molecular biology


liquid (aqueous solution)


pkg of 0.4 mL (08724105001)
pkg of 1.0 mL (08724121001)
pkg of 5 × 1.0 mL (08724156001)




transfection: suitable

storage temp.


General description

X-tremeGENE 360 Transfection Reagent is an innovative reagent that forms a complex with DNA or RNA and efficiently delivers the nucleic acids into animal or insect cells. With improved utility, X-tremeGENE 360 delivers high efficiency transfection in more cell types with low cytotoxicity.


X-tremeGENE 360 Transfection Reagent is a high performance transfection reagent, free of animal-derived components.
  • Designed to transfect a broad range of eukaryotic cells, many cell lines not transfected well by other reagents, and hard-to-transfect cell lines, such as asK-562 and HepG2.
  • Can be successfully used in a variety of applications, such as gene expression analysis using transiently transfected cells, generating of stable cell lines, expression of siRNA for gene knockdown studies, and CRISPR gene-editing tools.
  • Produces minimal cytotoxicity or changes in morphology when adequate numbers of cells are transfected, eliminating the requirement to change media after adding the transfection complex.
  • Suitable for transient and stable transfection.
  • Functions well in the presence or absence of serum.

Features and Benefits

X-tremeGENE 360 Transfection Reagent is a high-performing, versatile and reliable solution for hard-to-transfect and common cell types. As a proven and reliable reagent, X-tremeGENE 360 allows researchers to avoid guesswork when performing transfection. In addition, X-tremeGENE 360 exhibits:
  • Peak performance in a broad range of cell types
  • Suitable for multiple applications
  • Efficient delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 components


Each lot of X-tremeGENE 360 Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE 360 Transfection Reagent (ratio 3:1 μL/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin. Number of Tests: Using the standard procedure, 1 mL of X-tremeGENE 360 Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche


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Signal Word


Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids



Flash Point(F)

61.7 °F

Flash Point(C)

16.5 °C

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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Product Information Sheet


Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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