White precipitation occurs due to the formation of paraformaldehyde, a common phenomenon in formaldehyde solutions. This product contains 10-15% methanol as a stabilizer to slow down the process. Ideally, storage should be at 25°C, as lower temperatures accelerate polymerization. The formation of paraformaldehyde is reversible, so the product may still be usable. To achieve a clear solution, gently heat the cloudy solution up to 30°C for 1 to 2 hours, taking care not to overheat, as this can cause degradation.
252549
Formaldehyde solution
ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
Synonym(s):
Formalin
Sign Into View Organizational & Contract Pricing
Select a Size
25 ML
$55.90
500 ML
$62.70
1 L
$112.00
4 X 100 ML
$196.00
4 L
$323.00
6 X 500 ML
$529.00
All Photos(6)
Select a Size
Change View
25 ML
$55.90
500 ML
$62.70
1 L
$112.00
4 X 100 ML
$196.00
4 L
$323.00
6 X 500 ML
$529.00
About This Item
Linear Formula:
HCHO
CAS Number:
Molecular Weight:
30.03
Beilstein:
1209228
MDL number:
UNSPSC Code:
12352114
PubChem Substance ID:
NACRES:
NA.21
Recommended Products
grade
ACS reagent
Quality Level
vapor density
1.03 (vs air)
vapor pressure
52 mmHg ( 37 °C)
52 mmHg ( 37 °C)
form
liquid
autoignition temp.
572 °F
contains
10-15% Methanol as stabilizer (to prevent polymerization)
concentration
36.5-38.0%
37 wt. % in H2O
impurities
≤0.006 meq/g Titr. acid
ign. residue
≤0.005%
color
APHA: ≤10
refractive index
n20/D 1.3765
solubility
water: soluble
density
1.09 g/mL at 25 °C (lit.)
anion traces
chloride (Cl-): ≤5 ppm
sulfate (SO42-): ≤0.002%
cation traces
Fe: ≤5 ppm
heavy metals: ≤5 ppm (by ICP-OES)
suitability
suitable for manufacturing use (Laboratory chemicals)
SMILES string
[H]C([H])=O
InChI
1S/CH2O/c1-2/h1H2
InChI key
WSFSSNUMVMOOMR-UHFFFAOYSA-N
Looking for similar products? Visit Product Comparison Guide
Related Categories
1 of 4
This Item | 47608 | 15512 | F8775 |
|---|---|---|---|
| solubility water: soluble | solubility - | solubility - | solubility - |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| refractive index n20/D 1.3765 | refractive index - | refractive index - | refractive index - |
| density 1.09 g/mL at 25 °C (lit.) | density 1.09 g/mL at 20 °C, 1.09 g/mL at 25 °C (lit.) | density 1.09 g/mL at 25 °C (lit.) | density 1.09 g/mL at 25 °C (lit.) |
| form liquid | form liquid | form - | form liquid |
| grade ACS reagent | grade for molecular biology | grade - | grade for molecular biology |
General description
Application
Formaldehyde solution has been used as a fixing agent to fix cells during immunofluorescence imaging and for cross-linking cells during chromatin immunoprecipitation (ChIP) assay.[3]
Physical form
This product is a solution of approximately 37% by weight of formaldehyde gas in water.
related product
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3
Target Organs
Eyes,Central nervous system, Respiratory system
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Flash Point(F)
132.8 °F - closed cup
Flash Point(C)
56 °C - closed cup
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Intracellular protein and nucleic acid measured in eight cell types using deep-ultraviolet mass mapping.
Cheung MC et al.
Cytometry, 83, 540-540 (2013)
Mitochondrial translocation of alpha-synuclein is promoted by intracellular acidification.
Cole NB et al.
Experimental Cell Research, 314, 2076-2076 (2008)
Slug contributes to cancer progression by direct regulation of ERa signaling pathway.
Li Y et al.
International Journal of Oncology, 46, 1461-1461 (2015)
Farrell RE
RNA Methodologies: A Laboratory Guide for Isolation and Characterization (2010)
SIRT6 protects against endothelial dysfunction and atherosclerosis in mice.
Xu S et al.
Aging (Albany. NY.), 8, 1064-1064 (2016)
-
Hi! My formaldehyde solution presents a white precipitate, and I think it can be paraformaldehyde. Can it still be used as formaldehyde, or is there a way to redisolve it and make it functional again? Thanks!
1 answer-
Helpful?
-
-
How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
-
-
Can you store formaldehyde in plastic?
1 answer-
This product is packaged in glass bottles. Generally, formaldehyde solution can be used in plastic containers. However, it is advised that the unused material be preserved in its original packaging.
Helpful?
-
-
What type of packaging for different packages?
1 answer-
This product is packaged in amber glass bottles with polypropylene caps with HDPE liners. The bottle sizes are as follows:
252549-25 ML in 50 ml bottles
252549-100 ML in 100 ml bottles
252549-500ML in 500 ml bottles
252549-1L in 1L bottles
252549- 4L in 4L bottlesHelpful?
-
-
Can formaldehyde solution 37% be diluted to 10% formalin for fixing cells for staining? Do you dilute it with around 2.7 part water? Thank you!
1 answer-
How much water should be added to 100mL of a 37% solution to reduce its strength to a 10% solution?
Use C1 x V1= C2 x V2
37% x 100ml = 10% x V2
Therefore V2 = (37% x 100ml) / 10%
= 370 mLSubtract the total original volume from the new volume.
370 mL - 100 mL = 270 mL
So to reduce 100mL of 37% to 10% solution, add 270 mL of water to 100 mL of Formaldehyde solution - 252549-25ML.Helpful?
-
-
Which formaldehyde solution is recommended for use with the HT102A Reticulum kit?
1 answer-
When a silver stain procedure recommends a 10% formalin solution, it is referring to a 10% aqueous formalin solution, which is prepared by diluting 1 part of 37% to 40% formaldehyde with 9 parts deionized water. For histology, it is advisable to use ACS reagents when the product meets ACS Specifications. Suitable products for this purpose would be product numbers 252549 or FX0410. If a 37% to 40% formaldehyde solution is available, it should work; however, it's important to avoid using old bottles of this solution that have been in the lab for several years, as impurities can form over time. Solutions used in histological procedures should typically be replaced every 2 years to ensure effectiveness.
Helpful?
-
-
Is the methanol content in the CoA expressed as volume percent or weight percent?
1 answer-
The methanol content is expressed as weight percent.
Helpful?
-
-
What is the difference between Formaldehyde solution Products 252549 and 533998?
1 answer-
The two products are the same chemically. However, the 252549 is designated as an ACS reagent grade. With this description additional specifications are included.
Helpful?
-
-
How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
