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77730
Gram Staining Kit
suitable for microscopy, used for differentiation of bacteria on the basis of their gram nature.
Gram Staining Kit
Synonym(s):
Bacteria Staining Kit according to Gram
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1 KIT
$279.00
About This Item
NACRES:
NA.85
UNSPSC Code:
41171621
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Product Name
Gram Staining Kit, suitable for microscopy, used for differentiation of bacteria on the basis of their gram nature.
agency
according to GB 4789.30-2016
product line
BioChemika
shelf life
limited shelf life, expiry date on the label
technique(s)
microbe id | staining: suitable
suitability
suitable for microscopy
bacteria
antibiotic activity spectrum
Gram-negative bacteria
Gram-positive bacteria
application(s)
clinical testing
environmental
food and beverages
pharmaceutical
Quality Level
1 of 4
This Item | 1.01603 | 75482 | SCT071 |
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) Gram staining: suitable | technique(s) microbe id | staining: suitable | technique(s) - |
| Quality Level 100 | Quality Level 400 | Quality Level 100 | Quality Level - |
| grade for microscopy | grade - | grade - | grade - |
| agency according to GB 4789.30-2016 | agency - | agency - | agency - |
| product line BioChemika | product line - | product line - | product line - |
| shelf life limited shelf life, expiry date on the label | shelf life - | shelf life - | shelf life - |
Application
The Gram staining method is one of the most important staining techniques in microbiology. It is also the most important criteria in any identification scheme for all types of bacterial isolates to differentiate the gram-positive and gram-negative bacteria.
General description
The Gram staining kit is used in differential staining technique that is most widely applied in all microbiology disciplines laboratories. It is one of the most important criteria in any identification scheme for all types of bacterial isolates. There are many physiological differences between gram-positive and gram-negative cell walls. Ever since Christian Gram has discovered Gram staining, this process has been extensively investigated and redefined. In practice, a thin smear of bacterial cells is stained with crystal violet, then treated with iodine containing mordant to increase the binding of the primary stain. A decolorizing solution of alcohol or acetone is used to remove the crystal violet from cells which bind it weakly and then the counterstain safranin is used to provide color contrast in those cells that are decolorized. In a properly stained smear by gram staining procedure, the gram-positive bacteria appear blue to purple and gram-negative cells appear pink to red
Other Notes
Kit contains (250ml each):
94448 Gram′s crystal violet Solution
90107 Gram′s iodine Solution
75482 Gram′s Decolorizer Solution
94635 Gram′s safranin Solution
94448 Gram′s crystal violet Solution
90107 Gram′s iodine Solution
75482 Gram′s Decolorizer Solution
94635 Gram′s safranin Solution
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
target_organs
Central nervous system
supp_hazards
Storage Class
3 - Flammable liquids
flash_point_f
68.0 °F
flash_point_c
20 °C
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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