MilliporeSigma
All Photos(5)

B0770

Sigma-Aldrich

Brilliant Blue G

pure

Synonym(s):
Coomassie Brilliant Blue G, Acid blue 90
Empirical Formula (Hill Notation):
C47H48N3NaO7S2
CAS Number:
Molecular Weight:
854.02
Colour Index Number:
42655
Beilstein:
5230822
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

≥80.0% (HPLC)

form

powder

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=CC(\C=C5C)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1

InChI key

RWVGQQGBQSJDQV-UHFFFAOYSA-M

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Application

Brilliant Blue G has been used for determining the urine protein concentration. It has been used for the protein band detection after SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). It has been used as a P2X7R (purinergic receptor) antagonist.

Packaging

5, 25, 100 g in glass bottle

Biochem/physiol Actions

P2X7 purinergic receptor antagonist.
Brilliant Blue G is used for staining protein(s) in polyacrylamide gels. It is also a P2X7 purinergic receptor antagonist. The receptor is mainly involved in neuroinflammatory events. In human SOD1G93A amyotrophic lateral sclerosis mouse, administration of brilliant blue G reduces body weight loss. However, it does not affect survival or motor coordination in transgenic mice.

Legal Information

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Neuronal Release of Cytokine IL-3 Triggered by Mechanosensitive Autostimulation of the P2X7 Receptor Is Neuroprotective.
Lim JC, et al.
Frontiers in Cellular Neuroscience, 10, 270-270 (2016)
MATE transport of the E. coli-derived genotoxin colibactin.
Mousa JJ, et al.
Nature microbiology, 1, 15009-15009 (2016)
P2X7 antagonism using Brilliant Blue G reduces body weight loss and prolongs survival in female SOD1G93A amyotrophic lateral sclerosis mice.
PeerJ, e3064-e3064 (2017)
Melatonin attenuates hypertension-induced renal injury partially through inhibiting oxidative stress in rats.
Qiao YF, et al.
Molecular Medicine Reports, 13, 21-21 (2016)
COMPARATIVE EVALUATION OF SOMATIC PROTEINS OF THE NYMPHAL STAGE OF LINGUATULA SERRATA ISOLATED FROM GOATS AND CATTLE IN NORTHWEST IRAN.
Bulgarian Journal of Veterinary Medicine, 19, 242-246 (2016)

Protocols

Hand-casting gels for PAGE and SDS-PAGE using TurboMix™ Bis-Tris Gel Casting Kits

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using TurboMix™ Bis-Tris Gel Casting Kits

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