This product is Collagenase Type IV, which digests type IV collagen, a major structural component of the basement membrane. It is likely that this product will affect the extracellular matrix to some extent. This product has not been tested for this specific application. Reviewing literature on this topic may provide additional insights.
C4-BIOC
Collagenase Type IV, Cls IV
Synonym(s):
Collagen A, Type IV Gelatinase
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100 MG
$79.40
1 G
$470.00
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100 MG
$79.40
1 G
$470.00
About This Item
UNSPSC Code:
12352204
NACRES:
NA.78
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General description
Collagenase Type IV, Cls IV is a member of the mammalian extracellular neutral metalloproteinases family. It digests type IV collagen, which is a major structural constituent of the basement membrane.[1]
This product is identical to Biochrom GmbH part numbers C4-22 and C4-28. Enzyme blending from collagenase, clostripain, with tryptic and proteolytic activities. Recommended for the isolation of Islets of Langerhans. Specific activity is 125 to 250 Mandl units per milligram of powdered substance.
Application
Collagenase Type IV, Cls IV may be used:
- as a component in Dulbecco′s modified Eagle′s medium (DMEM)/F12 to digest endometrial samples isolated from in vitro fertilization (IVF) patients[2]
- for collagenase perfusion to isolate hepatocytes using collagenase-liver perfusion method in rats[3]
- in the isolation of rat glomerular endothelial cells (GEndCs)[4]
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Hyun-Jeong Cho et al.
Biological & pharmaceutical bulletin, 28(8), 1418-1423 (2005-08-05)
Microsomal triglyceride (TG) transfer protein (MTP) is involved in the secretion of TG-rich very low-density lipoprotein (VLDL), a process which leads to the generation of hypertriglyceridemia and atherosclerosis. We investigated the possible role of Ca(2+) on MTP activity in hepatocytes.
P Huhtala et al.
The Journal of biological chemistry, 265(19), 11077-11082 (1990-07-05)
The structure of the gene for human 70-kDa type IV collagenase (gelatinase) was determined. Three overlapping genomic clones were isolated and shown to contain 0.4 kilobase (kb) of the 5'-flanking region, the 27-kb structural gene, and 4.5 kb of the
Mingtai Gui et al.
Experimental and therapeutic medicine, 22(6), 1446-1446 (2021-11-02)
Continuous and irreversible cardiac hypertrophy can induce cardiac maladaptation and cardiac remodeling, resulting in increased risk of developing cardiovascular diseases. The present study was conducted to investigate the therapeutic effect of Huoxue Qianyang Qutan recipe (HQQR) on angiotensin II (Ang
Zongliang Zhang et al.
Journal of translational medicine, 21(1), 23-23 (2023-01-13)
Chimeric antigen receptor (CAR) T cells and immune checkpoint blockades (ICBs) have made remarkable breakthroughs in cancer treatment, but the efficacy is still limited for solid tumors due to tumor antigen heterogeneity and the tumor immune microenvironment. The restrained treatment
Rui-Xian Liu et al.
iScience, 26(3), 106140-106140 (2023-03-08)
Antibody-secreting B cells have long been considered the central element of gut homeostasis; however, tumor-associated B cells in human colorectal cancer (CRC) have not been well characterized. Here, we show that the clonotype, phenotype, and immunoglobulin subclasses of tumor-infiltrating B
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Does it detach the collagen IV without damaging the ECM or affecting the structure of it?
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What is the storage condition and shelf life after reconstituting the product?
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The long-term stability of this product in solution has not been determined. However, a similar Collagenase type IV product, C5138, notes that solutions are stable for several months when stored in aliquots at -20°C. Please see the link below to review the datasheet of a similar item:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/380/876/c5138pis.pdfHelpful?
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How does one solubilize collagenase, and what is the usage concentration for digestion and cell isolation?
1 answer-
The substance is soluble at 50mg/ml and can be dissolved in water, although it is recommended to use saline, NaCl, DPBS, Hanks, or a culture medium. It is worth noting that in peer-reviewed papers at sial.com, glomerular endothelial cells were isolated at 0.1% in DMEM.
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